免疫胶体金渗滤法快速定量检测食品中噻虫嗪残留

Immunochromatographic test strip for quantitative and rapid detection of tolfenpyrad in food samples.

机构信息

International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China.

出版信息

J Chromatogr B Analyt Technol Biomed Life Sci. 2023 Aug 1;1228:123837. doi: 10.1016/j.jchromb.2023.123837. Epub 2023 Jul 22.

DOI:10.1016/j.jchromb.2023.123837

PMID:37524012

Abstract

In the study, a hapten was designed to preserve the molecular structure of tolfenpyrad while introducing a carboxyl group and was coupled with a carrier protein to synthesize an immunogen and coating antigen. A monoclonal antibody was fabricated against tolfenpyrad and its performance was assessed by indirect competitive enzyme-linked immunosorbent assay. Finally, we developed a colloidal gold nanoparticle immunochromatographic test strip (CGN-ICTS) for the detection of tolfenpyrad in kale, Chinese cabbage, and eggplant samples. The results shows that CGN-ICTS was sensitive, with calculated detection limits of 0.49 ng/g for kale and Chinese cabbage and 0.99 ng/g for eggplant. Subsequently, CGN-ICTS and LC-MS were used to analyze the tolfenpyrad-spiked samples. The recovery rate of the CGN-ICTS for kale samples was 97.1-103.0%, for Chinese cabbage samples was 93.7-103.4%, and for eggplant samples was 92.7-105.7%. Recovery rates were similar between CGN-ICTS and LC-MS. Therefore, CGN-ICTS can be used to quickly screen tolfenpyrad residues in foods.

摘要

在这项研究中，设计了一种半抗原，以保留噻虫啉的分子结构，同时引入羧基，并与载体蛋白偶联以合成免疫原和包被抗原。针对噻虫啉制备了单克隆抗体，并通过间接竞争酶联免疫吸附试验评估了其性能。最后，我们开发了一种胶体金免疫层析测试条（CGN-ICTS），用于检测羽衣甘蓝、白菜和茄子样品中的噻虫啉。结果表明，CGN-ICTS 具有较高的灵敏度，对羽衣甘蓝和白菜的检测限分别为 0.49ng/g 和 0.99ng/g，对茄子的检测限为 0.99ng/g。随后，使用 CGN-ICTS 和 LC-MS 分析了噻虫啉加标样品。CGN-ICTS 对羽衣甘蓝样品的回收率为 97.1-103.0%，对白菜样品的回收率为 93.7-103.4%，对茄子样品的回收率为 92.7-105.7%。CGN-ICTS 与 LC-MS 的回收率相似。因此，CGN-ICTS 可用于快速筛选食品中的噻虫啉残留。