Wang Peng, Xu Xinxin, Liu Liqiang, Song Shanshan, Kuang Hua, Xu Chuanlai, Wu Xiaoling
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, People's Republic of China.
International Joint Research Laboratory for Biointerface and Biodetection and School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, People's Republic of China.
J Food Sci. 2022 Oct;87(10):4538-4547. doi: 10.1111/1750-3841.16288. Epub 2022 Sep 14.
In this study, an excellent monoclonal antibody (mAb) was produced that could sensitively and specifically recognize Flu in fruit samples. The mAb belonged to the IgG2 subclass and had a half-inhibitory concentration (IC ) of 0.59 ng/ml and a limit of detection (LOD) of 0.13 ng/ml. A cross-reactivity test revealed that the mAb had good specificity for Flu. A gold nanoparticle-based lateral-flow immunochromatographic (ICA) strip was used for the rapid screening of Flu in fruit samples. The method had a visual cut-off value of 10 µg/kg for Flu in three different fruit samples when evaluated with the naked eye. The LODs were 5.25, 3.13, and 4.64 µg/kg for Flu in apples, grapes, and oranges, respectively, as obtained with the aid of a strip scan reader. Therefore, this ICA strip assay represented a potential tool for the detection of Flu in fruit samples.
在本研究中，制备了一种出色的单克隆抗体（mAb），它能够灵敏且特异性地识别水果样品中的氟虫腈。该单克隆抗体属于IgG2亚类，半抑制浓度（IC）为0.59 ng/ml，检测限（LOD）为0.13 ng/ml。交叉反应试验表明，该单克隆抗体对氟虫腈具有良好的特异性。基于金纳米颗粒的侧向流动免疫色谱（ICA）条带用于水果样品中氟虫腈的快速筛查。当用肉眼评估时，该方法对三种不同水果样品中氟虫腈的目视临界值为10 μg/kg。借助条带扫描读取器，苹果、葡萄和橙子中氟虫腈的检测限分别为5.25、3.13和4.64 μg/kg。因此，这种ICA条带检测法是检测水果样品中氟虫腈的一种潜在工具。
