Ad Y, Halperin I M, Olstad E C, Gershwin L J, Sullivan L, Reagan K L
William R. Pritchard Veterinary Medical Teaching Hospital, University of California Davis, 1 Shields Ave., Davis 95616, CA, USA.
Department of Medicine and Epidemiology, University of California, School of Veterinary Medicine, Davis, 1 Shields Ave., Davis 95616, CA, USA.
Vet J. 2023 Aug-Sep;298-299:106017. doi: 10.1016/j.tvjl.2023.106017. Epub 2023 Jul 29.
Guidelines recommend that dogs are vaccinated for canine distemper virus (CDV), canine parvovirus (CPV), and canine adenovirus (CAV) every 3 years. Alternatively, their antibody titers are measured and vaccines given when titers fall below a protective threshold. In this study, a point-of-care (POC) assay was compared to hemagglutination inhibition (for CPV) and virus neutralization (for CAV and CDV) assays to predict the need for revaccination Ninety-two dogs presented for vaccination were enrolled. The POC assay indicated protective titers against CDV in 79/80, CPV in 89/90, and CAV in 91/91 dogs with reference standard antibody measurements that were over a protective threshold. The sensitivity of the POC assay for to detect protective concentrations of CDV antibodies was 99% (95% confidence interval [CI 95%], 93.3-99.9%). Ten dogs were falsely considered protected against CDV by the POC assay with a specificity of 17% (CI 95%, 3.0-44.8%). The sensitivity of the POC assay for protective concentrations of CPV titers was 99% (CI 95%, 93.9-99.9%). The sensitivity of the POC assay to detect protective concentrations of CAV antibodies was 100% (CI 95%, 95.9-100%). Only classifying high-positive CDV and CPV titers on the POC assay as protective improved assay specificity to 100%, but sensitivity decreased to 51% and 76% respectively. This POC assay had a high sensitivity for the detection of protective antibody titers; however, some dogs were falsely categorized as protected, especially for CDV.
指南建议犬类每3年接种犬瘟热病毒（CDV）、犬细小病毒（CPV）和犬腺病毒（CAV）疫苗。或者，检测它们的抗体滴度，当滴度降至保护阈值以下时进行疫苗接种。在本研究中，将一种即时检测（POC）方法与血凝抑制试验（用于CPV）和病毒中和试验（用于CAV和CDV）进行比较，以预测再次接种疫苗的必要性。纳入了92只前来接种疫苗的犬。POC检测显示，在80只犬中有79只针对CDV、90只犬中有89只针对CPV、91只犬中有91只针对CAV的抗体滴度具有保护作用，其参考标准抗体测量值超过保护阈值。POC检测检测CDV抗体保护浓度的敏感性为99%（95%置信区间[CI 95%]，93.3 - 99.9%）。有10只犬被POC检测错误地判定为对CDV有保护作用，特异性为17%（CI 95%，3.0 - 44.8%）。POC检测CPV滴度保护浓度的敏感性为99%（CI 95%，93.9 - 99.9%）。POC检测检测CAV抗体保护浓度的敏感性为100%（CI 95%，95.9 - 100%）。仅将POC检测中高阳性的CDV和CPV滴度分类为具有保护作用，可将检测特异性提高到100%，但敏感性分别降至51%和76%。这种POC检测在检测保护性抗体滴度方面具有较高的敏感性；然而，一些犬被错误地归类为具有保护作用，尤其是对于CDV。
