Departament d`Estomatologia, Facultat de Medicina i Odontologia, Universitat de Valencia, Gascó Oliag, 1, Valencia 46010, Spain.
Departament d`Estomatologia, Facultat de Medicina i Odontologia, Universitat de Valencia, Gascó Oliag, 1, Valencia 46010, Spain.
J Dent. 2023 Sep;136:104645. doi: 10.1016/j.jdent.2023.104645. Epub 2023 Jul 29.
To identify the metabolomic differences in the saliva of healthy children versus children with active carious lesions and to estimate the predictive capacity of a model based on the salivary metabolomic profile.
A study of cases (n = 31) and controls (n = 37) was designed for children aged between 6 and 12 (mean age of the cases: 8.9; controls: 8.7). The said children attended public health centers in Valencia, Spain. Intraoral examinations were performed by a single examiner using ICDAS II diagnostic criteria. Unstimulated total saliva samples were analyzed by nuclear magnetic resonance (NMR) spectroscopy.
The dft index for cases was 2.84 while it was 0.19 for the control group, the DMFT index was 1.13 and 0.11, respectively. The predictive model generated by the multivariate PLS-DA analysis projects a separation between the cases and the controls on the score chart with a predictive capacity and generating an area under the curve of 0.71. The metabolites: 3-methyl-2-oxovalerate, 3-hydroxybutyrate, lactate, acetone, citrate, ornithine, ethanolamine, taurine, proline, glycine, mannose, glucose, 1-6-Anhydro-β-d-glucose and citraconate, are those that show greater significance in the model. In the controls, glycine (Cohen's d = 0.430) and glucose (Cohen's d = 0.560) present higher means compared to the cases. On the contrary, taurine (Cohen's d= -0.474) and mannose (Cohen's d= -0.456) show higher means in cases compared to controls.
Our findings show a difference in the salivary metabolomic profiles, specifically in the groups of saccharides and amino acids, suggesting an association of these with the level of caries risk.
The results reported in the present study reinforce the use of salivary metabolomics as a research method for the search for salivary biomarkers that allow the evaluation of caries risk in patients. Furthermore, it brings us closer to a personalized medicine that will help in dental caries prevention strategies.
确定健康儿童与患有活动性龋病儿童的唾液代谢组学差异，并评估基于唾液代谢组学特征的模型的预测能力。
设计了一项病例（n=31）和对照（n=37）研究，纳入年龄在 6 至 12 岁之间的儿童（病例组平均年龄：8.9 岁；对照组：8.7 岁）。这些儿童在西班牙瓦伦西亚的公共卫生中心就诊。由一名检查者使用 ICDAS II 诊断标准进行口腔内检查。对非刺激性全唾液样本进行核磁共振（NMR）光谱分析。
病例组的 dft 指数为 2.84，而对照组为 0.19；DMFT 指数分别为 1.13 和 0.11。多元偏最小二乘判别分析（PLS-DA）生成的预测模型在得分图上对病例和对照组进行了区分，其预测能力产生的曲线下面积为 0.71。在模型中具有更大意义的代谢物有：3-甲基-2-氧代戊酸、3-羟基丁酸、乳酸、丙酮、柠檬酸、鸟氨酸、乙醇胺、牛磺酸、脯氨酸、甘氨酸、甘露糖、葡萄糖、1-6-脱水-β-d-葡萄糖和柠康酸。在对照组中，与病例组相比，甘氨酸（Cohen's d=0.430）和葡萄糖（Cohen's d=0.560）的平均值更高。相反，牛磺酸（Cohen's d=-0.474）和甘露糖（Cohen's d=-0.456）在病例组中的平均值高于对照组。
我们的研究结果表明唾液代谢组图谱存在差异，特别是在糖和氨基酸组，这表明这些代谢物与龋病风险水平有关。
本研究报告的结果证实了唾液代谢组学作为寻找唾液生物标志物的研究方法的应用，这些生物标志物可用于评估患者的龋病风险。此外，它使我们更接近个性化医疗，这将有助于龋病预防策略。
