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评价致病变种大肠杆菌致实验性猪尿路感染的尿液试纸检测。

Evaluation of urine dipstick tests in experimental porcine urinary tract infection with uropathogenic Escherichia coli.

机构信息

Department of Clinical Microbiology, Odense University Hospital, Odense, Denmark.

Research Unit of Clinical Microbiology, University of Southern Denmark, Odense, Denmark.

出版信息

Sci Rep. 2023 Jul 31;13(1):12404. doi: 10.1038/s41598-023-39239-7.

DOI:10.1038/s41598-023-39239-7
PMID:37524716
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10390499/
Abstract

Urinary tract infection is a common disease in pigs and a major reason for sows to be culled. The disease, however, is difficult to diagnose due to lack of distinct clinical signs in the animals. We evaluated the diagnostic value of two commercial urine dipstick tests in 10 pigs using an experimental model of Escherichia coli urinary tract infection. Urine collected at baseline and 48 h after inoculation were analyzed. We show that dipstick tests positive of blood, leucocytes and particularly nitrite are very specific for E. coli UTI with a 100% positive predictive value.

摘要

尿路感染是猪的常见病,也是母猪淘汰的主要原因。然而,由于动物缺乏明显的临床症状,因此该病难以诊断。我们使用大肠杆菌尿路感染的实验模型评估了两种商业尿液试纸检测在 10 头猪中的诊断价值。分析了基线和接种后 48 小时采集的尿液。我们表明,尿液试纸检测血液、白细胞和特别是亚硝酸盐阳性对大肠杆菌尿路感染具有非常高的特异性,阳性预测值为 100%。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9acc/10390499/743458dab66f/41598_2023_39239_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9acc/10390499/1369d46283b7/41598_2023_39239_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9acc/10390499/8d4eecb7ab07/41598_2023_39239_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9acc/10390499/743458dab66f/41598_2023_39239_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9acc/10390499/1369d46283b7/41598_2023_39239_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9acc/10390499/8d4eecb7ab07/41598_2023_39239_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9acc/10390499/743458dab66f/41598_2023_39239_Fig3_HTML.jpg

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Evaluation of urine dipstick tests in experimental porcine urinary tract infection with uropathogenic Escherichia coli.评价致病变种大肠杆菌致实验性猪尿路感染的尿液试纸检测。
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本文引用的文献

1
Clean-catching urine from pigs: A method for collecting quality specimens for urinalysis and microbiological culturing in a laboratory environment.从猪采集清洁尿样:一种在实验室环境下收集尿液分析和微生物培养用高质量标本的方法。
Lab Anim. 2023 Jun;57(3):293-303. doi: 10.1177/00236772221133433. Epub 2022 Dec 1.
2
Intracellular uropathogenic Escherichia coli are undetectable in urinary bladders after oral mecillinam treatment: An experimental study in a pig model of cystitis.口服美西林治疗后,尿路上皮细胞内尿路致病性大肠埃希菌不可检测:膀胱炎猪模型的实验研究。
Microb Pathog. 2022 Dec;173(Pt A):105817. doi: 10.1016/j.micpath.2022.105817. Epub 2022 Oct 14.
3
Genome-wide analysis of fitness factors in uropathogenic Escherichia coli in a pig urinary tract infection model.
尿路致病性大肠杆菌在猪尿路感染模型中适应性因子的全基因组分析。
Microbiol Res. 2022 Dec;265:127202. doi: 10.1016/j.micres.2022.127202. Epub 2022 Sep 15.
4
Uropathogenic can cause cystitis at extremely low inocula in a pig model.尿路致病性 在猪模型中极低接种量即可引起膀胱炎。
J Med Microbiol. 2022 Apr;71(4). doi: 10.1099/jmm.0.001537.
5
type-1 fimbriae are critical to overcome initial bottlenecks of infection upon low-dose inoculation in a porcine model of cystitis.1 型菌毛对于克服低剂量接种诱导膀胱炎猪模型中感染的初始瓶颈至关重要。
Microbiology (Reading). 2021 Oct;167(10). doi: 10.1099/mic.0.001101.
6
A Novel Device-Integrated Drug Delivery System for Local Inhibition of Urinary Tract Infection.一种用于局部抑制尿路感染的新型装置集成药物递送系统。
Front Microbiol. 2021 Jun 25;12:685698. doi: 10.3389/fmicb.2021.685698. eCollection 2021.
7
Urinary tract infections in culled sows from Greek herds: prevalence and associations between findings of histopathology, bacteriology and urinalysis.希腊猪群中淘汰母猪的尿路感染:组织病理学、细菌学和尿液分析结果之间的患病率及关联
Porcine Health Manag. 2021 Apr 19;7(1):33. doi: 10.1186/s40813-021-00212-3.
8
Diagnosis of endometritis and cystitis in sows: use of biomarkers.母猪子宫内膜炎和膀胱炎的诊断:生物标志物的应用。
J Anim Sci. 2020 Aug 18;98(Suppl 1):S107-S116. doi: 10.1093/jas/skaa144.
9
A Porcine Model for Urinary Tract Infection.一种用于尿路感染的猪模型。
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Clin Infect Dis. 2019 May 2;68(10):e83-e110. doi: 10.1093/cid/ciy1121.