State Key Laboratory of Chemical Resource Engineering, College of Chemistry, Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing University of Chemical Technology, Beijing 100029, P. R. China.
Institute of Chemistry, Chinese Academy of Sciences, Zhongguancun North First Street 2, 100190 Beijing, P. R. China.
Anal Chem. 2023 Aug 15;95(32):11943-11952. doi: 10.1021/acs.analchem.3c01447. Epub 2023 Aug 1.
Schizophrenia is a common mental disorder with unclear mechanisms. Oxidative stress and neuroinflammation play important roles in the pathological process of schizophrenia. Superoxide anion (O) is an important oxidative stress biomarker . However, due to the existence of the blood-brain barrier (BBB), few near-infrared (NIR) fluorescent probes have been used for the sensing and detection of O in the brain. With this research, we developed the first near-infrared fluorescent probe (named CT-CF) for noninvasive detection of endogenous O in the brain of mice. Enabling fluorescence monitoring of the dynamic changes in O flux due to the prolonged activation of microglia in neuroinflamed and schizophrenic (SZ) mice brains, thereby providing direct evidence for the relationship between oxidative stress, neuroinflammation, and schizophrenia. Furthermore, we confirmed the O burst in the brains of first-episode schizophrenic mice and assessed the effect of two atypical antipsychotic drugs (risperidone and olanzapine) on redox homeostasis.
精神分裂症是一种常见的精神障碍，其发病机制尚不清楚。氧化应激和神经炎症在精神分裂症的病理过程中起着重要作用。超氧阴离子（O）是一种重要的氧化应激生物标志物。然而，由于血脑屏障（BBB）的存在，很少有近红外（NIR）荧光探针用于大脑中 O 的传感和检测。通过这项研究，我们开发了第一个用于非侵入性检测小鼠大脑内内源性 O 的近红外荧光探针（命名为 CT-CF）。该探针能够荧光监测由于神经炎症和精神分裂症（SZ）小鼠大脑中小胶质细胞的长时间激活而导致的 O 通量的动态变化，从而为氧化应激、神经炎症和精神分裂症之间的关系提供了直接证据。此外，我们还证实了首发精神分裂症小鼠大脑中的 O 爆发，并评估了两种非典型抗精神病药物（利培酮和奥氮平）对氧化还原平衡的影响。
