Olsen J D, Ralphs M H
Am J Vet Res. 1986 Aug;47(8):1829-33.
Twelve Hereford heifers were infused with larkspur extract (group 1, n = 4), lithium chloride solution (group 2, n = 4), or sodium chloride solution (group 3, n = 4). After 3 weeks of being fed basal ration (meadow grass hay), all heifers were offered a ration of alfalfa pellets (3,400 g) in a 2-phase, single-day feeding regimen. In phase 1, the amount eaten within 45 minutes was measured by weigh-back of uneaten pellets. In phase 2, cattle that ate greater than 150 g of pellets in phase 1 were offered the balance of uneaten pellets and were infused with their respective designated solution. Five pairings of pellet feedings and appropriate infusions were given at 2- or 3-day intervals to initially induce feed aversion. Heifers in groups 1 and 2 developed a strong aversion to pellets by the 5th feeding. Persistence of aversion was tested twice at weekly intervals. Infusion of cattle with larkspur extract or lithium chloride solution induced a persistent aversion to an otherwise palatable feed.
12头赫里福德小母牛分别被注入了飞燕草提取物（第1组，n = 4）、氯化锂溶液（第2组，n = 4）或氯化钠溶液（第3组，n = 4）。在以基础日粮（草地干草）喂养3周后，所有小母牛在一个两阶段单日喂养方案中被给予苜蓿颗粒料（3400克）。在第1阶段，通过称取未食用颗粒料的重量来测量45分钟内的采食量。在第2阶段，在第1阶段采食超过150克颗粒料的牛被给予剩余未食用的颗粒料，并被注入各自指定的溶液。每隔2天或3天进行5次颗粒料喂食和适当注射，以初步诱导采食厌恶。第1组和第2组的小母牛在第5次喂食时对颗粒料产生了强烈的厌恶。每隔一周对厌恶的持续性进行两次测试。给牛注入飞燕草提取物或氯化锂溶液会导致对原本可口的饲料产生持续性厌恶。
