Chandler D B, Kennedy J I, Fulmer J D
Am Rev Respir Dis. 1986 Sep;134(3):542-7. doi: 10.1164/arrd.1986.134.3.542.
Recent data suggest that pulmonary macrophages may be heterogeneous. Several studies have demonstrated that alveolar macrophages are functionally and biochemically heterogeneous. In addition, interstitial macrophages, which are believed to be the precursors to alveolar macrophages, have been suggested to be heterogeneous. Therefore, this study was undertaken to determine if density-defined interstitial macrophages (DD-IM) are heterogeneous with respect to receptors for zymosan, immunoglobulin, and complement as well as morphologically. Furthermore, avidity for IgG was defined by opsonizing sheep red blood cells (SRBC) with different amounts of IgG. Interstitial macrophages were harvested and separated into 18 DD-IM subpopulations by centrifugation through a continuous iso-osmotic gradient of colloidal silica. Interstitial macrophages showed marked heterogeneity in cellular volume. Furthermore, macrophages of density 1.046 to 1.075 g/ml exhibited higher receptor activity capability of attaching and phagocytizing SRBC opsonized with small amounts of IgG and towards zymosan. All DD-IM exhibited similar abilities to attach complement-coated SRBC. These results demonstrate the functional heterogeneity of interstitial macrophages with respect to IgG and zymosan.
近期数据表明肺巨噬细胞可能具有异质性。多项研究已证实肺泡巨噬细胞在功能和生化方面具有异质性。此外，据信是肺泡巨噬细胞前体的间质巨噬细胞也被认为具有异质性。因此，开展本研究以确定密度界定的间质巨噬细胞（DD - IM）在酵母聚糖、免疫球蛋白和补体受体方面以及形态上是否具有异质性。此外，通过用不同量的IgG调理绵羊红细胞（SRBC）来定义对IgG的亲和力。收获间质巨噬细胞，并通过在连续等渗胶体二氧化硅梯度中离心将其分离为18个DD - IM亚群。间质巨噬细胞在细胞体积上表现出明显异质性。此外；密度为1.046至1.075 g/ml的巨噬细胞对用少量IgG调理的SRBC以及对酵母聚糖表现出更高的附着和吞噬受体活性能力。所有DD - IM在附着补体包被的SRBC方面表现出相似的能力。这些结果证明了间质巨噬细胞在IgG和酵母聚糖方面功能的异质性。
