Shanghai Key Lab for Urban Ecological Processes and Eco-Restoration, School of Ecological and Environmental Sciences, East China Normal University, Shanghai, 200241, PR China.
Shanghai Key Lab for Urban Ecological Processes and Eco-Restoration, School of Ecological and Environmental Sciences, East China Normal University, Shanghai, 200241, PR China; Shanghai Engineering Research Center of Biotransformation of Organic Solid Waste, Shanghai, 200241, PR China; Chongqing Key Laboratory of Precision Optics, Chongqing Institute of East China Normal University, Chongqing, 401120, China; Institute of Eco-Chongming (IEC), 3663 N. Zhongshan Rd, Shanghai, 200062, PR China.
Chemosphere. 2023 Oct;339:139676. doi: 10.1016/j.chemosphere.2023.139676. Epub 2023 Jul 30.
The disintegration of anaerobic sludge and blockage of membrane pores has impeded the practical application of anaerobic membrane bioreactor (AnMBR) in treating methanolic wastewater. In this study, bioelectrochemical system (BES) was integrated into AnMBR to alleviate sludge dispersion and membrane fouling as well as enhance bioconversion of methanol. Bioelectrochemical regulation effect induced by BES enhanced methane production rate from 4.94 ± 0.52 to 5.39 ± 0.37 L/L/d by accelerating the enrichment of electroactive microorganisms and the agglomeration of anaerobic sludge via the adhesive and chemical bonding force. 16 S rRNA gene high-throughput sequencing demonstrated that bioelectrochemical stimulation had modified the metabolic pathways by regulating the key functional microbial communities. Methanogenesis via the common methylotrophic Methanomethylovorans was partially substituted by the hydrogenotrophic Candidatus_Methanofastidiosum, etc. The metabolic behaviors of methanol are bioelectrochemistry-dependent, and controlling external voltage is thus an effective strategy for ensuring robust electron transfer, low membrane fouling, and long-term process stability.
厌氧污泥的解体和膜孔堵塞阻碍了厌氧膜生物反应器（AnMBR）在处理甲醇废水方面的实际应用。在本研究中，生物电化学系统（BES）被集成到 AnMBR 中，以减轻污泥分散和膜污染，并增强甲醇的生物转化。BES 引起的生物电化学调节效应通过加速电活性微生物的富集和厌氧污泥的聚集，通过粘附和化学键力，将甲烷产生速率从 4.94±0.52 L/L/d 提高到 5.39±0.37 L/L/d。16S rRNA 基因高通量测序表明，生物电化学刺激通过调节关键功能微生物群落，改变了代谢途径。通过常见的甲基营养型 Methanomethylovorans 的甲烷生成过程部分被氢营养型 Candidatus_Methanofastidiosum 等取代。甲醇的代谢行为依赖于生物电化学，因此控制外部电压是确保电子传递稳健、膜污染低和长期过程稳定的有效策略。
