Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University , Sapporo, Hokkaido, Japan.
The Institute of Scientific and Industrial Research, Osaka University, Mihogaoka, Ibaraki-shi, Osaka, Japan.
Biosci Biotechnol Biochem. 2023 Sep 21;87(10):1129-1138. doi: 10.1093/bbb/zbad102.
Squalene is a triterpenoid compound and widely used in various industries such as medicine and cosmetics due to its strong antioxidant and anticancer properties. The purpose of this study is to increase the accumulation of squalene in filamentous fungi using exogeneous butenafine hydrochloride, which is an inhibitor for squalene epoxidase. The detailed settings achieved that the filamentous fungi, Trichoderma virens PS1-7, produced squalene up to 429.93 ± 51.60 mg/L after culturing for 7 days in the medium consisting of potato infusion with glucose at pH 4.0, in the presence of 200 µm butenafine. On the other hand, no squalene accumulation was observed without butenafine. This result indicated that squalene was biosynthesized in the filamentous fungi PS1-7, which can be used as a novel source of squalene. In addition, we successfully obtained highly 13C-enriched squalene by using [U-13C6]-glucose as a carbon source replacing normal glucose.
角鲨烯是一种三萜类化合物，由于其具有很强的抗氧化和抗癌特性，广泛应用于医药和化妆品等各个行业。本研究旨在通过添加角鲨烯环氧化酶抑制剂丁烯氟虫腈来增加丝状真菌中角鲨烯的积累。详细的设置实现了，在 pH 值为 4.0 的土豆浸液葡萄糖培养基中培养 7 天后，丝状真菌绿色木霉 PS1-7 产生的角鲨烯高达 429.93±51.60mg/L，同时存在 200µM 的丁烯氟虫腈。另一方面，没有丁烯氟虫腈就观察不到角鲨烯的积累。这一结果表明，角鲨烯是在 PS1-7 丝状真菌中生物合成的，可以作为角鲨烯的一种新型来源。此外，我们成功地使用[U-13C6]-葡萄糖作为碳源替代正常葡萄糖，获得了高度 13C 富集的角鲨烯。
