Department of Cardiology, Nanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, Nanjing, 210022, China.
Department of Nephrology, the Key Laboratory for the Prevention and Treatment of Chronic Kidney Disease of Chongqing, Chongqing Clinical Research Center of Kidney and Urology Diseases, Xinqiao Hospital, Army Medical University (Third Military Medical University), Chongqing, 400037, China.
Chin J Integr Med. 2023 Dec;29(12):1077-1086. doi: 10.1007/s11655-023-3642-z. Epub 2023 Aug 2.
To reveal the anti-inflammatory mechanism of Guanxin V, which is prescribed for ventricular remodeling in clinical practice.
Guanxin V-, ventricular remodeling-, and inflammation-related targets were obtained through an integrated strategy of virtual screening and systematic pharmacology, and then the shared targets were visualised with a Venn diagram. Guanxin V network and the protein-protein interaction network were drawn, and enrichment analysis was conducted. Finally, the main results obtained from the integrated strategy were validated by molecular docking and in vivo experiments.
A total of 251, 11,425, and 15,246 Guanxin V-, ventricular remodeling-, and inflammation-related targets were acquired, respectively. Then, 211 shared targets were considered to contribute to the mechanism of ventricular remodeling treated by Guanxin V. Guanxin network and the protein-protein interaction network were drawn, and enrichment analysis showed some cardiovascular-related biological processes and signaling pathways. Molecular docking revealed that the Guanxin V-derived compounds could align with key targets. Final in vivo experiments proved that Guanxin V reverses ventricular remodeling by inhibiting inflammation.
Guanxin V relieves ventricular remodeling by regulating inflammation, which provides new ideas for the anti-ventricular remodeling mechanism of Guanxin V.
揭示临床上用于心室重构的冠心 V 的抗炎机制。
通过虚拟筛选和系统药理学的综合策略获得冠心 V、心室重构和炎症相关靶点，然后用韦恩图可视化共有靶点。绘制冠心 V 网络和蛋白质-蛋白质相互作用网络，并进行富集分析。最后，通过分子对接和体内实验验证综合策略获得的主要结果。
分别获得了 251、11425 和 15246 个冠心 V、心室重构和炎症相关靶点。然后，认为 211 个共有靶点有助于冠心 V 治疗心室重构的机制。绘制了冠心网络和蛋白质-蛋白质相互作用网络，富集分析显示了一些心血管相关的生物学过程和信号通路。分子对接表明，冠心 V 衍生的化合物可以与关键靶点结合。最终的体内实验证明，冠心 V 通过抑制炎症来逆转心室重构。
冠心 V 通过调节炎症缓解心室重构，为冠心 V 的抗心室重构机制提供了新的思路。
