• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

吡啶基离子液体的合成及其在甲醇-水溶剂体系中提高脂肪酶稳定性的应用。

Synthesis of pyridinium-based ionic liquids and their application to improve lipase stability in a methanol-water solvent system.

作者信息

Hendra Cipta Oktavianus, Alni Anita, Hertadi Rukman

机构信息

Department of Chemistry, Bandung Institute of Technology, Bandung, Indonesia.

出版信息

Turk J Chem. 2022 Oct 31;47(2):307-320. doi: 10.55730/1300-0527.3539. eCollection 2023.

DOI:10.55730/1300-0527.3539
PMID:37528930
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10388036/
Abstract

This paper studied the effect of pyridinium-based ionic liquids as cosolvents in a methanol-water solvent system on the hydrolytic activity of lipase. These ionic liquids were successfully synthesized using imidazolium-based ionic liquid synthesizing methods with a certain adjustment. The hydrolytic activity of lipase was analyzed using 4-nitrophenol acetate (pNPA) and 4-nitrophenol palmitate (pNPP) as substrates. The addition of ionic liquids had no significant effect on the hydrolytic activity of lipase in a water solvent, and it had a greater effect in methanol. The addition of [C6Py] Br ionic liquid as a methanol cosolvent (methanol: ionic liquid, 10:5) could increase the hydrolytic activity of lipase. The use of ionic liquid as a cosolvent could increase the hydrolytic activity of lipase by about 15.61% while using pNPP as a substrate in the methanol system. A molecular dynamics study for the interaction between lipase and ionic liquids supported the experimental results. The ionic liquid using bromide as an anion provided more stability on lipase conformation. It tends to form the short-range interaction between the lipase and bromide anion.

摘要

本文研究了吡啶基离子液体作为共溶剂在甲醇 - 水溶剂体系中对脂肪酶水解活性的影响。这些离子液体采用咪唑基离子液体合成方法并做了一定调整后成功合成。以乙酸对硝基苯酯（pNPA）和棕榈酸对硝基苯酯（pNPP）为底物分析脂肪酶的水解活性。在水溶剂中添加离子液体对脂肪酶的水解活性没有显著影响，而在甲醇中影响较大。添加[C6Py]Br离子液体作为甲醇共溶剂（甲醇：离子液体，10:5）可提高脂肪酶的水解活性。在甲醇体系中以pNPP为底物时，使用离子液体作为共溶剂可使脂肪酶的水解活性提高约15.61%。对脂肪酶与离子液体之间相互作用的分子动力学研究支持了实验结果。以溴化物作为阴离子的离子液体对脂肪酶构象提供了更高的稳定性。它倾向于在脂肪酶和溴离子之间形成短程相互作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ac7/10388036/b45782dad309/turkjchem-47-2-307f14.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ac7/10388036/cab7d9375da9/turkjchem-47-2-307f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ac7/10388036/de02f8063f9b/turkjchem-47-2-307f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ac7/10388036/7e7b50081132/turkjchem-47-2-307f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ac7/10388036/9fd7465818ad/turkjchem-47-2-307f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ac7/10388036/06f935eae90d/turkjchem-47-2-307f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ac7/10388036/e4b42df1e88b/turkjchem-47-2-307f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ac7/10388036/662938e4ec9f/turkjchem-47-2-307f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ac7/10388036/2db2e95892f2/turkjchem-47-2-307f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ac7/10388036/0b81d1ad3935/turkjchem-47-2-307f9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ac7/10388036/8f71f91e7d28/turkjchem-47-2-307f10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ac7/10388036/3cc8b9191ac1/turkjchem-47-2-307f11.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ac7/10388036/bb95322a417a/turkjchem-47-2-307f12.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ac7/10388036/bd94758f04f3/turkjchem-47-2-307f13.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ac7/10388036/b45782dad309/turkjchem-47-2-307f14.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ac7/10388036/cab7d9375da9/turkjchem-47-2-307f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ac7/10388036/de02f8063f9b/turkjchem-47-2-307f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ac7/10388036/7e7b50081132/turkjchem-47-2-307f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ac7/10388036/9fd7465818ad/turkjchem-47-2-307f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ac7/10388036/06f935eae90d/turkjchem-47-2-307f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ac7/10388036/e4b42df1e88b/turkjchem-47-2-307f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ac7/10388036/662938e4ec9f/turkjchem-47-2-307f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ac7/10388036/2db2e95892f2/turkjchem-47-2-307f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ac7/10388036/0b81d1ad3935/turkjchem-47-2-307f9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ac7/10388036/8f71f91e7d28/turkjchem-47-2-307f10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ac7/10388036/3cc8b9191ac1/turkjchem-47-2-307f11.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ac7/10388036/bb95322a417a/turkjchem-47-2-307f12.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ac7/10388036/bd94758f04f3/turkjchem-47-2-307f13.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ac7/10388036/b45782dad309/turkjchem-47-2-307f14.jpg

相似文献

1
Synthesis of pyridinium-based ionic liquids and their application to improve lipase stability in a methanol-water solvent system.吡啶基离子液体的合成及其在甲醇-水溶剂体系中提高脂肪酶稳定性的应用。
Turk J Chem. 2022 Oct 31;47(2):307-320. doi: 10.55730/1300-0527.3539. eCollection 2023.
2
Ionic liquids as a reaction medium for lipase-catalyzed methanolysis of sunflower oil.离子液体作为脂肪酶催化葵花籽油甲醇解反应的介质
Biotechnol Lett. 2007 Dec;29(12):1881-5. doi: 10.1007/s10529-007-9471-x. Epub 2007 Jul 19.
3
Chemical modification for improving catalytic performance of lipase B from Candida antarctica with hydrophobic proline ionic liquid.用疏水性脯氨酸离子液体对南极假丝酵母脂肪酶 B 进行化学修饰以提高其催化性能。
Bioprocess Biosyst Eng. 2022 Apr;45(4):749-759. doi: 10.1007/s00449-022-02696-x. Epub 2022 Feb 3.
4
Kinetic resolution of ibuprofen catalyzed by Candida rugosa lipase in ionic liquids.皱褶假丝酵母脂肪酶在离子液体中催化布洛芬的动力学拆分
Chirality. 2005 Jan;17(1):16-21. doi: 10.1002/chir.20078.
5
Increased enantioselectivity and remarkable acceleration of lipase-catalyzed transesterification by using an imidazolium PEG-alkyl sulfate ionic liquid.通过使用咪唑鎓聚乙二醇烷基硫酸盐离子液体提高脂肪酶催化酯交换反应的对映选择性并显著加速反应。
Chemistry. 2006 Dec 13;12(36):9228-37. doi: 10.1002/chem.200601043.
6
Impact of ionic liquid physical properties on lipase activity and stability.离子液体物理性质对脂肪酶活性和稳定性的影响。
J Am Chem Soc. 2003 Apr 9;125(14):4125-31. doi: 10.1021/ja028557x.
7
Chemical modification for improving activity and stability of lipase B from Candida antarctica with imidazolium-functional ionic liquids.用咪唑基功能化离子液体对南极假丝酵母脂肪酶 B 进行化学修饰以提高其活性和稳定性。
Org Biomol Chem. 2013 Nov 7;11(41):7192-8. doi: 10.1039/c3ob41076e.
8
Thermal stability enhancements of Candida rugosa lipase in ionic liquids.离子液体中皱褶假丝酵母脂肪酶的热稳定性增强
Commun Agric Appl Biol Sci. 2003;68(2 Pt A):293-6.
9
Chemical modification with functionalized ionic liquids: a novel method to improve the enzymatic properties of Candida rugosa lipase.功能化离子液体的化学修饰：一种改善皱褶假丝酵母脂肪酶酶学性质的新方法。
Bioprocess Biosyst Eng. 2014 Aug;37(8):1617-26. doi: 10.1007/s00449-014-1134-4. Epub 2014 Feb 4.
10
Enhancing bio-catalytic activity and stability of lipase nanogel by functional ionic liquids modification.通过功能化离子液体修饰提高脂肪酶纳米凝胶的生物催化活性和稳定性。
Colloids Surf B Biointerfaces. 2020 Nov;195:111275. doi: 10.1016/j.colsurfb.2020.111275. Epub 2020 Jul 24.

本文引用的文献

1
Molecular mechanism of enzyme tolerance against organic solvents: Insights from molecular dynamics simulation.酶耐受有机溶剂的分子机制：来自分子动力学模拟的见解。
Int J Biol Macromol. 2019 Feb 1;122:914-923. doi: 10.1016/j.ijbiomac.2018.10.172. Epub 2018 Oct 26.
2
First-principles and Molecular Dynamics simulation studies of functionalization of Au golden fullerene with amino acids.基于第一性原理和分子动力学模拟研究氨基酸官能化 Au 金富勒烯。
Sci Rep. 2018 Jul 30;8(1):11400. doi: 10.1038/s41598-018-29887-5.
3
Ultrasound-assisted chemoenzymatic epoxidation of soybean oil by using lipase as biocatalyst.超声辅助脂肪酶作为生物催化剂的大豆油环氧化酶促反应。
Ultrason Sonochem. 2018 Jan;40(Pt A):912-920. doi: 10.1016/j.ultsonch.2017.08.042. Epub 2017 Sep 1.
4
A convenient test for lipase activity in aqueous-based solutions.一种用于检测水基溶液中脂肪酶活性的便捷测试方法。
Enzyme Microb Technol. 2015 Apr;71:8-12. doi: 10.1016/j.enzmictec.2015.01.005. Epub 2015 Jan 25.
5
Structural and dynamic features of Candida rugosa lipase 1 in water, octane, toluene, and ionic liquids BMIM-PF6 and BMIM-NO3.在水中、辛烷、甲苯和离子液体 BMIM-PF6 和 BMIM-NO3 中，Candida rugosa 脂肪酶 1 的结构和动态特征。
J Phys Chem B. 2013 Mar 7;117(9):2662-70. doi: 10.1021/jp312299d. Epub 2013 Feb 27.
6
Esterification activity and conformation studies of Burkholderia cepacia lipase in conventional organic solvents, ionic liquids and their co-solvent mixture media.在常规有机溶剂、离子液体及其共溶剂混合介质中，洋葱伯克霍尔德氏菌脂肪酶的酯化活性和构象研究。
Bioresour Technol. 2010 Dec;101(24):9822-4. doi: 10.1016/j.biortech.2010.07.107. Epub 2010 Aug 1.
7
Bacterial biocatalysts: molecular biology, three-dimensional structures, and biotechnological applications of lipases.细菌生物催化剂：脂肪酶的分子生物学、三维结构及生物技术应用
Annu Rev Microbiol. 1999;53:315-51. doi: 10.1146/annurev.micro.53.1.315.
8
VMD: visual molecular dynamics.VMD：可视化分子动力学
J Mol Graph. 1996 Feb;14(1):33-8, 27-8. doi: 10.1016/0263-7855(96)00018-5.
9
On the structural stability and solvent denaturation of proteins. I. Denaturation by the alcohols and glycols.关于蛋白质的结构稳定性和溶剂变性。I. 醇类和二醇类引起的变性
J Biol Chem. 1970 May 25;245(10):2588-98.