Zhang Tiefeng, Gan Yaxin, Bai Yulan, Hao Weijiang, Zeng Ziqi, Wu Feng, Li Xianqi
Shanxi Medical University School and Hospital of Stomatology, Shanxi Province Key Laboratory of Oral Diseases Prevention and New Materials, Taiyuan, Shanxi, 030001, China.
Department of Oral and Maxillofacial Surgery, School of Dentistry, Matsumoto Dental University, Shiojiri, 399-0781, Japan.
Biochem Biophys Rep. 2023 Jul 28;35:101523. doi: 10.1016/j.bbrep.2023.101523. eCollection 2023 Sep.
Bell's palsy is the most common form of facial nerve palsy. This study aimed to explore the pathogenesis of Bell's palsy by investigating the effect of cold-stimulated adipocyte supernatant on adhesion molecule expression in Schwann cell line. Schwann cells were cultured in regular or adipocyte-conditioned medium and analyzed using RNA sequencing. The mRNA expression of Schwann cell adhesion molecules melanoma cell adhesion molecule (MCAM), protocadherin 9 (PCDH9), and intercellular cell adhesion molecule 1 (ICAM1) was determined using real-time reverse-transcription polymerase chain reaction. Differentially expressed genes were identified, and Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses were conducted. Compared with Schwann cells in 37 °C, the expression of MCAM, PCDH9, and ICAM1 was downregulated in Schwann cells treated with cold-stimulated adipocyte supernatant compared with Schwann cells in 37 °C. Adipocytes subjected to cold exposure may weaken the adhesion capacity of Schwann cells and disrupt the local homeostasis of Schwann cell-axon interactions by affecting the expression of MCAM, PCDH9, and ICAM1, ultimately leading to the development of demyelinating lesions.
贝尔面瘫是面神经麻痹最常见的形式。本研究旨在通过研究冷刺激脂肪细胞上清液对雪旺细胞系中黏附分子表达的影响，探讨贝尔面瘫的发病机制。将雪旺细胞培养于常规培养基或脂肪细胞条件培养基中，并采用RNA测序进行分析。使用实时逆转录聚合酶链反应测定雪旺细胞黏附分子黑素瘤细胞黏附分子（MCAM）、原钙黏蛋白9（PCDH9）和细胞间黏附分子1（ICAM1）的mRNA表达。鉴定差异表达基因，并进行基因本体论和京都基因与基因组百科全书通路富集分析。与37℃培养的雪旺细胞相比，用冷刺激脂肪细胞上清液处理的雪旺细胞中MCAM、PCDH9和ICAM1的表达下调。冷暴露的脂肪细胞可能会削弱雪旺细胞的黏附能力，并通过影响MCAM、PCDH9和ICAM1的表达破坏雪旺细胞-轴突相互作用的局部稳态，最终导致脱髓鞘病变的发生。
