Zhang Dong, Li ZongYing, Gao Yuan, Sun HaiLing
The First Department of Nephrology, Cangzhou Central Hospital, Hebei Province, China.
Department of Hematology, Cangzhou Central Hospital, Hebei Province, China.
Turk J Biol. 2022 Dec 9;47(1):61-73. doi: 10.55730/1300-0152.2641. eCollection 2023.
From (Fisch.) Bge.var. mongholicus (Bge.) Hsiao, astragaloside IV (AS-IV), a saponin can be purified and is considered traditional Chinese medicine. The purpose of this study was to evaluate the AS-IV-mediated mechanism on chronic glomerulonephritis (CGN). A cationic bovine serum albumin-induced CGN rat model was established and 10, 15, or 20 mg/kg of AS-IV was administered to measure renal function and inflammatory infiltration. Influences of AS-IV on proliferation, cell cycle, and inflammation of LPS-induced rat mesangial cells (RMCs) were determined. The results demonstrated that AS-IV alleviated renal dysfunction, renal lesions, and inflammation in CGN rats. AS-IV prolonged the G0-G1 phase, shortened the S phase, and inhibited cell proliferation and inflammation in RMCs. AS-IV can promote miR-181d-5p expression to inhibit CSF1. miR-181d-5p promotion or CSF1 suppression could further enhance the therapeutic role of AS-IV in CGN rats, while miR-181d-5p silencing or CSF1 overexpression abolished the effect of AS-IV. In conclusion, AS-IV by mediating the miR-181d-5p/CSF1 axis protects against CGN.
从蒙古黄芪（Fisch.）Bge.var. mongholicus（Bge.）Hsiao中可纯化得到黄芪甲苷IV（AS-IV），它是一种皂苷，被视为传统中药。本研究旨在评估AS-IV对慢性肾小球肾炎（CGN）的作用机制。建立阳离子牛血清白蛋白诱导的CGN大鼠模型，并给予10、15或20mg/kg的AS-IV，以检测肾功能和炎症浸润情况。测定AS-IV对脂多糖诱导的大鼠系膜细胞（RMCs）增殖、细胞周期和炎症的影响。结果表明，AS-IV可减轻CGN大鼠的肾功能障碍、肾损伤和炎症。AS-IV延长了G0-G1期，缩短了S期，并抑制了RMCs的细胞增殖和炎症。AS-IV可促进miR-181d-5p表达以抑制CSF1。促进miR-181d-5p表达或抑制CSF1可进一步增强AS-IV对CGN大鼠的治疗作用，而沉默miR-181d-5p或过表达CSF1则可消除AS-IV的作用。总之，AS-IV通过介导miR-181d-5p/CSF1轴对CGN起到保护作用。
