Geldof A A, Rao B R, de Voogt H J
Anticancer Res. 1986 Jul-Aug;6(4):837-40.
A semisolid agar assay to determine the number of colony forming cells in monodispersed cell suspensions of the rat prostate hormone independent tumor variant R3327-Mat Lu was established. Using this technique the effects of several chemotherapeutic agents upon the clonogenic potential of the tumor cells were investigated. Adriamycin and cisplatinum proved to be effective in reducing the number of colonies formed, in a dose dependent manner. The combined, sequential incubation with adriamycin and cisplatinum did not have a synergistic effect. Cyclophosphamide. Hydroxyurea, 5-fluorouracil and methotrexate were not effective in the reduction of the number of colonies in vitro at concentrations as high as 500 micrograms/ml. Interestingly treatment with these agents increased the number of colonies formed, compared to control.
建立了一种半固体琼脂试验，用于测定大鼠前列腺激素非依赖性肿瘤变体R3327-Mat Lu单分散细胞悬液中集落形成细胞的数量。使用该技术研究了几种化疗药物对肿瘤细胞克隆形成潜力的影响。阿霉素和顺铂被证明能以剂量依赖的方式有效减少形成的集落数量。阿霉素和顺铂联合、序贯孵育没有协同作用。环磷酰胺、羟基脲、5-氟尿嘧啶和甲氨蝶呤在浓度高达500微克/毫升时，在体外减少集落数量方面无效。有趣的是，与对照相比，用这些药物处理增加了形成的集落数量。
