deficiency causes male sterility and testis abnormal in .

INTRODUCTION

It has been established that encodes E3 ubiquitin ligase, which determines the specificity of substrate binding during protein ubiquitination and has been associated with various functions of the nervous system but not the reproductive system. Herein, we explored the role of on fertility with a .

METHODS

Different knockdown flies were established using the UAS/GAL4 activating sequence system. Fertility, hatchability, and testis morphology were studied, and bioinformatics analyses were conducted. Our results indicated that deficiency could induce male sterility and influent egg hatchability in .

RESULTS

We found that deficiency affected the testis during morphological analysis. Proteomics analysis indicated 188 upregulated proteins and 175 downregulated proteins in the testis of knockdown flies. Gene Ontology analysis revealed significant upregulation of and , and downregulation of in knockdown flies. These proteins were involved in the biometabolic or reproductive process in . These regulated proteins are important in testis generation and sperm storage promotion. Bioinformatics analysis verified that was low expressed in cryptorchidism patients, which further supported the important role of in male fertility.

DISCUSSION

Overall, our findings suggest that deficiency could promote male infertility and may be involved in the protein modification of by upregulating and , whereas downregulating Pelota protein expression.