（分析前）考虑到对滑液钙卫蛋白进行分析。

(Pre)analytical considerations concerning the analysis of synovial calprotectin.

机构信息

Department of Laboratory Medicine, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.

Department of Medical Microbiology and Infection Prevention, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.

出版信息

Clin Chem Lab Med. 2023 Aug 3;62(1):199-206. doi: 10.1515/cclm-2023-0484. Print 2024 Jan 26.

DOI:10.1515/cclm-2023-0484

PMID:37529863

Abstract

OBJECTIVES

Several studies have demonstrated that synovial calprotectin is a highly accurate biomarker in diagnosing periprosthetic joint infections (PJI). Assuring reliability is of great importance and coincides with adequate preanalytical handling. This study focuses on potentially interfering factors.

METHODS

To assess the stability of synovial calprotectin, the effect of time, storage temperature, EDTA, freeze-thaw cycles, viscosity, and blood and lipid contamination was investigated. In the blood and lipid contamination experiments, hemolyzed and non-hemolyzed blood, homogenized adipose tissue, intralipid and chylomicrons were added. The effect of viscosity was investigated using freeze-thaw cycles, enzymatic pretreatment and sonification.

RESULTS

No effect on synovial calprotectin levels was observed in synovial samples kept at room temperature compared to samples kept at 4 °C for up to seven days of storage. Freeze-thaw cycles did not result in significantly different calprotectin levels, although samples without EDTA resulted in higher recoveries after 1 and 2 freeze-thaw cycles. Blood and lipid contamination did not interfere with accurate synovial calprotectin analysis. Sample pretreatment to reduce sample viscosity by pretreating samples with DNAse and/or hyaluronidase did not influence calprotectin analysis. Sonification, however, resulted in increased calprotectin values.

CONCLUSIONS

Synovial calprotectin is a stable biomarker and its analysis is not easily influenced by potential interfering factors.

摘要

目的

多项研究表明，滑膜钙卫蛋白是诊断人工关节假体周围感染（PJI）的一种高度准确的生物标志物。确保可靠性非常重要，这与充分的分析前处理是一致的。本研究侧重于潜在的干扰因素。

方法

为了评估滑膜钙卫蛋白的稳定性，考察了时间、储存温度、EDTA、冻融循环、黏度以及血液和脂质污染对其的影响。在血液和脂质污染实验中，加入了溶血和非溶血血液、均质脂肪组织、Intralipid 和乳糜微粒。通过冻融循环、酶预处理和超声处理来研究黏度的影响。

结果

与储存在 4°C 下长达 7 天的样本相比，室温下储存的滑膜样本中滑膜钙卫蛋白水平没有变化。冻融循环不会导致钙卫蛋白水平显著不同，尽管没有 EDTA 的样本在 1 和 2 次冻融循环后回收量更高。血液和脂质污染不会干扰准确的滑膜钙卫蛋白分析。通过用 DNAse 和/或透明质酸酶预处理样本以降低样本黏度的样品预处理不会影响钙卫蛋白分析。然而，超声处理会导致钙卫蛋白值升高。