The Biotechnology & Drug Development Research Laboratory, Curtin Medical School & Curtin Health Innovation Research Institute, Curtin University, Bentley 6102, Perth, Western Australia, Australia.
Hearing Therapeutics Department, Ear Science Institute Australia, Queen Elizabeth II Medical Centre, Nedlands 6009, Perth, Western Australia, Australia.
Nanomedicine (Lond). 2023 May;18(12):923-940. doi: 10.2217/nnm-2023-0092. Epub 2023 Aug 2.
Sensorineural hearing loss has been associated with oxidative stress. However, an antioxidant that passes effectively through the ear remains elusive. Probucol (PB)-based nanoparticles were formed using a spray-drying encapsulation technique, characterized and tested . Uniform, spherical nanoparticles were produced. The addition of lithocholic acid to PB formulations did not affect drug content or production yield, but it did modify capsule size, surface tension, electrokinetic stability and drug release. Cell viability, bioenergetics and inflammatory profiles were improved when auditory cells were exposed to PB-based nanoparticles, which showed antioxidant properties (p < 0.05). PB-based nanoparticles can potentially protect the auditory cell line from oxidative stress and could be used in future studies as a potential new therapeutic agent for sensorineural hearing loss.
感音神经性听力损失与氧化应激有关。然而，一种能有效穿透耳朵的抗氧化剂仍然难以捉摸。采用喷雾干燥包封技术形成了以丙丁酚（PB）为基础的纳米颗粒，并对其进行了表征和测试。生成了均匀的球形纳米颗粒。在 PB 制剂中添加石胆酸不会影响药物含量或生产收率，但会改变胶囊大小、表面张力、动电学稳定性和药物释放。当听觉细胞暴露于具有抗氧化性能的 PB 纳米颗粒时，细胞活力、生物能量和炎症特征得到改善（p<0.05）。PB 纳米颗粒可能有潜力保护听觉细胞免受氧化应激，未来可能作为治疗感音神经性听力损失的潜在新治疗剂进行研究。
