Ann Ital Chir. 2023;93:316-321.
AIM - Anastomotic leakage is among the most common complications following gastrointestinal surgery. MATERIAL AND METHODS - This study aimed to determine the effects of stem cells and platelet-rich fibrin (PRF) on anastomotic healing. The study included 60 rats that were randomly divided into 3 groups, each with 2 subgroups. The study included the control group (no treatment post anastomosis), the PRF group (PRF administered following anastomosis), and the PRF + stem cell group (PRF + stem cells administered following anastomosis). Anastomosis was performed at the descending colon in all groups. Anastomosis bursting pressure was determined, and histopathological and angiographic examination were performed on postoperative D 7. RESULTS - Intraabdominal adhesion was significantly more common in the control group. Anastomosis bursting pressure was significantly higher and angiogenesis was significantly more common in the PRF + stem cell group (P < 0.005). Based on histopathologic examination, vascular proliferation and inflammation were significantly more common in the PRF + stem cell group than in the control group (P < 0.005). CONCLUSION : In cases of risky gastrointestinal system anastomosis, PRF + stem cells might reduce the incidence of anastomotic healing. KEY WORDS: Anastomosis, Platelet-rich fibrin, Stem cell, Wound healing.
目的 - 肠吻合口漏是胃肠道手术后最常见的并发症之一。
材料和方法 - 本研究旨在确定干细胞和富血小板纤维蛋白（PRF）对吻合口愈合的影响。该研究纳入了 60 只大鼠，随机分为 3 组，每组又分为 2 个亚组。对照组（吻合后不治疗）、PRF 组（吻合后给予 PRF）和 PRF+干细胞组（吻合后给予 PRF+干细胞）。所有组均在降结肠进行吻合。在术后第 7 天测定吻合口爆裂压，并进行组织病理学和血管造影检查。
结果 - 对照组的腹腔内粘连明显更常见。PRF+干细胞组的吻合口爆裂压显著升高，血管生成也更常见（P<0.005）。根据组织病理学检查，PRF+干细胞组的血管增生和炎症明显比对照组更常见（P<0.005）。
结论 - 在胃肠道系统吻合存在风险的情况下，PRF+干细胞可能会降低吻合口愈合的发生率。
吻合，富血小板纤维蛋白，干细胞，伤口愈合。
