Polysorbate 80 surface modified PLGA nanoparticles: an evaluation of cellular uptake and cytotoxicity on neuro-2a cells.

AIM

Present study focuses on the development of P80 coated PLGA Nanoparticles loaded with drugs, paroxetine (P80-Par-PLGA-NPs) and clonidine (P80-CLD-PLGA-NPs) for evaluation of Cellular Uptake & Cytotoxicity on Neuro-2a cells.

METHOD

P80-Par-PLGA-NPs and P80-CLD-PLGA-NPs were developed and characterised for zeta size, potential, PDI, EE%, DL%, TEM, SEM, FTIR, DSC, i release, cytotoxicity, histopathological and cell uptake studies using rhodamine loaded P80-NPs.

RESULT

Mean particle diameter of P80-Par-PLGA-NPs and P80-CLD-PLGA-NPs was 204; 182.7 nm, ZP of -21.8; -18.72 mV and 0.275; 0.341 PDI, respectively. TEM and SEM images revealed homogenous surface morphology. drug release showed sustained and complete release in 72 h. Cell viability (>90%) at C and no cytotoxicity in histopathology was observed. Significant higher uptake (96.9%) of P80-modified-NPS was observed as compared to unmodified-NPs (81%) (p < 0.05).

CONCLUSION

The finding clearly indicated a higher cell uptake of drugs via surface modified P80-coated PLGA-NPs as compared to unmodified particles.