Campos E, McConathy W J
Arch Biochem Biophys. 1986 Sep;249(2):455-63. doi: 10.1016/0003-9861(86)90022-6.
A study on the fractionation of human plasma into various lipoprotein classes was performed by single spin vertical ultracentrifugation. Detailed apolipoprotein (A-I, A-II, B, C-III, and E) and lipid (cholesterol, cholesterol ester, and triacylglycerol) analyses were performed on the fractions obtained by single spin vertical ultracentrifugation. ApoB was located primarily in the low-density lipoprotein (LDL) fraction whereas Lp(a) was detected in the fractions extending into the high-density lipoprotein region. The distributions of ApoA-I and ApoA-II varied among individuals, suggesting heterogeneity in the composition of HDL, and this was confirmed by the distributions of ApoC-III, ApoD, and ApoE. ApoC-III and ApoD were associated primarily with HDL3 while ApoE showed the most variation among subjects, spreading across the entire density spectrum. Distributions of cholesterol and cholesterol ester coincided with the elution of major apolipoproteins A-I and B while the distribution of triacylglycerol was variable. An immunoassay for lecithin:cholesterol acyltransferase (LCAT) demonstrated that the majority of LCAT was present in the high- to very high-density lipoprotein region, but LCAT was also detected in LDL and the immunoreactivity extended into the very low-density lipoprotein region. The presence of apolipoproteins A-I and A-II, as well as LCAT in the intermediate lipoprotein density region (LDL1) was consistent with these species representing lipolytic remnants. This study indicated that rapid (3 h) single spin vertical ultracentrifugation offers insights into the heterogeneity of lipoproteins and offers a useful tool for monitoring perturbations of the plasma lipid transport system. In conjunction with immunosorbers, immunoassays, and micro lipid analyses, this procedure offers a method to isolate and characterize various lipoprotein subspecies.
通过单旋垂直超速离心法对人血浆进行分离，得到不同的脂蛋白类别。对单旋垂直超速离心法获得的各组分进行详细的载脂蛋白（A-I、A-II、B、C-III和E）及脂质（胆固醇、胆固醇酯和三酰甘油）分析。载脂蛋白B主要存在于低密度脂蛋白（LDL）组分中，而脂蛋白（a）[Lp(a)]在延伸至高密度脂蛋白区域的组分中被检测到。载脂蛋白A-I和A-II的分布在个体间存在差异，提示高密度脂蛋白（HDL）组成存在异质性，载脂蛋白C-III、载脂蛋白D和载脂蛋白E的分布也证实了这一点。载脂蛋白C-III和载脂蛋白D主要与HDL3相关，而载脂蛋白E在受试者中变化最大，分布于整个密度谱。胆固醇和胆固醇酯的分布与主要载脂蛋白A-I和B的洗脱一致，而三酰甘油的分布则存在变化。卵磷脂胆固醇酰基转移酶（LCAT）免疫测定表明，大部分LCAT存在于高至极高密度脂蛋白区域，但在LDL中也检测到LCAT，其免疫反应性延伸至极低密度脂蛋白区域。中间密度脂蛋白区域（LDL1）中存在载脂蛋白A-I、载脂蛋白A-II以及LCAT，这与这些组分代表脂解残余物一致。本研究表明，快速（3小时）单旋垂直超速离心法有助于深入了解脂蛋白的异质性，并为监测血浆脂质转运系统的扰动提供了一个有用的工具。结合免疫吸附剂、免疫测定和微量脂质分析，该方法提供了一种分离和鉴定各种脂蛋白亚类的方法。
