长链非编码 RNA BRE-AS1 通过下调 miR-106b-5p 抑制透明细胞肾细胞癌的增殖、迁移和侵袭。

Long non-coding RNA BRE-AS1 inhibits proliferation, migration and invasion of clear cell renal cell carcinoma by downregulating miR-106b-5p.

机构信息

Department of Urology, the Second Affiliated Hospital of Nanchang University, Nanchang City, Jiangxi Province, PR China.

Department of Anesthesia, the Second Affiliated Hospital of Nanchang University, Nanchang City, Jiangxi Province, PR China.

出版信息

Histol Histopathol. 2024 Jun;39(6):729-737. doi: 10.14670/HH-18-653. Epub 2023 Jul 25.

DOI:10.14670/HH-18-653

PMID:37530129

Abstract

OBJECTIVE

The objective of this study was to investigate the involvement of the long non-coding RNA (lncRNA) BRE-AS1 in clear cell renal cell carcinoma (ccRCC) and to explore its potential therapeutic role.

METHODS

The expression of BRE-AS1 and miR-106b-5p was determined by qRT-PCR. Overexpression of BRE-AS1 and miR-106b-5p were performed to study their relationship. Transwell assays were used to evaluate cell movement. Methylation-specific PCR (MSP) was performed to explore the role of BRE-AS1 in the methylation of the miR-106b-5p gene.

RESULTS

The results showed that the expression levels of BRE-AS1 were decreased, while those of miR-106b-5p were increased in ccRCC tissues. BRE-AS1 was found to be closely associated with the prognosis of patients with ccRCC. The expression of BRE-AS1 was inversely correlated with that of miR-106b-5p in tumor tissues. Overexpression of BRE-AS1 led to decreased expression levels of miR-106b-5p and increased methylation of the miR-106b-5p gene, whereas miR-106b-5p did not affect the expression of BRE-AS1. BRE-AS1 inhibited the movement and proliferation of ccRCC cell lines, while miR-106b-5p suppressed the role of BRE-AS1.

CONCLUSION

BRE-AS1 may suppress ccRCC by downregulating the expression of miR-106b-5p.

摘要

目的

本研究旨在探讨长链非编码 RNA（lncRNA） BRE-AS1 在透明细胞肾细胞癌（ccRCC）中的作用，并探讨其潜在的治疗作用。

方法

采用 qRT-PCR 检测 BRE-AS1 和 miR-106b-5p 的表达。过表达 BRE-AS1 和 miR-106b-5p 以研究它们之间的关系。Transwell 实验评估细胞迁移。甲基化特异性 PCR（MSP）用于探索 BRE-AS1 在 miR-106b-5p 基因甲基化中的作用。

结果

结果表明，ccRCC 组织中 BRE-AS1 的表达水平降低，而 miR-106b-5p 的表达水平升高。BRE-AS1 与 ccRCC 患者的预后密切相关。肿瘤组织中 BRE-AS1 的表达与 miR-106b-5p 的表达呈负相关。过表达 BRE-AS1 导致 miR-106b-5p 表达水平降低，miR-106b-5p 基因甲基化增加，而 miR-106b-5p 不影响 BRE-AS1 的表达。BRE-AS1 抑制 ccRCC 细胞系的运动和增殖，而 miR-106b-5p 抑制 BRE-AS1 的作用。