Role of HLA alleles polymorphism in systemic lupus erythematosus: A prospective study from North India.

BACKGROUND

Systemic lupus erythematosus (SLE) is a chronic autoimmune disorder and has complex etiopathogenesis. The most appropriate hypothesis states that genetic susceptibility in the presence of environmental risk factors predisposes to SLE. HLA class II alleles are critical to immune response and are highly polymorphic. Various alleles in HLA-DR and -DQ regions were analyzed in SLE patients and healthy controls to see their role in susceptibility or protection to SLE.

MATERIALS AND METHODS

This was a prospective observational study, in which a total of 100 SLE patients and 100 controls were analyzed. HLA typing was done by polymerase chain reaction (PCR)-sequence-specific oligonucleotide (SSO) method (SSO probe).

RESULTS

DRβ10301 was significantly increased in SLE patients when compared to controls and had the highest odds ratio. Other risk factor alleles found to be increased were DRβ10701, DQβ10202, and DQβ10301, which had a significant positive association with SLE, suggesting their role in susceptibility to SLE. In contrast, DRβ10401, DRβ11401, DRβ11404, DRβ11501, DQβ10501, and DQα10201 showed statistically significant reduction in SLE patients, while these were much more common in controls, suggesting their protective role.

CONCLUSION

This study is only the second study in patients from North India and it determines the role of DRβ10301, DRβ10701, DQβ10202, and DQβ10301 alleles as risk factors in SLE patients.