Master's Degree Program in Environmental Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai, 50200, Thailand; Graduate School, Chiang Mai University, Chiang Mai, 50200, Thailand.
Energy Research and Development Institute - Nakornping, Chiang Mai University, Chiang Mai, 50200, Thailand.
Environ Res. 2023 Nov 1;236(Pt 2):116807. doi: 10.1016/j.envres.2023.116807. Epub 2023 Jul 31.
Effluent from biogas plants can contribute to serious environmental issues due to its high organic compound content and its dark color. This study utilizes Pseudomonas putida strains isolated from activated sludge in a wastewater treatment plant to reduce the organic compound content and dark color of biogas plant effluent. The treatment of effluent from the anaerobic digestion of distillery wastewater with P. putida in the best evaluated conditions (sample concentration of 50% v/v, agitation and aeration rates of 250 rpm and 1.0 vvm for 6 days) produced significant decolorization and organic removal efficacies of 34.29 ± 0.87% and 38.94 ± 0.63%, respectively. Bioremediation efficiency was dependent on bacterial growth, and the organic content and dissolved oxygen in the sample during cultivation were key factors in bacterial growth. The naturally selected bacterium could tolerate relatively high levels of organic content and work synergistically with other indigenous microorganisms found in the biogas plant effluent. Therefore, the bioremediation of biogas plant effluent using naturally selected P. putida can feasibly be applied in biogas power plants where sterilization is not necessary.
沼气厂的废水由于其高有机化合物含量和深色而可能对严重的环境问题产生贡献。本研究利用从污水处理厂活性污泥中分离出的恶臭假单胞菌（Pseudomonas putida）菌株来降低沼气厂废水的有机化合物含量和深色。在最佳评价条件下（样品浓度为 50%v/v、搅拌和曝气速率分别为 250 rpm 和 1.0 vvm，持续 6 天），用恶臭假单胞菌处理酒精厂废水的厌氧消化废水，产生了显著的脱色和有机去除效果，分别为 34.29±0.87%和 38.94±0.63%。生物修复效率取决于细菌的生长，而在培养过程中样品中的有机含量和溶解氧是细菌生长的关键因素。自然选择的细菌可以耐受相对较高的有机含量，并与沼气厂废水中发现的其他土著微生物协同作用。因此，使用自然选择的恶臭假单胞菌对沼气厂废水进行生物修复，可以在不需要灭菌的沼气发电厂中实际应用。
