UGT2B13 和 UGT2C1 参与了稻纵卷叶螟对 lambda-氯氰菊酯的抗性。
UGT2B13 and UGT2C1 are involved in lambda-cyhalothrin resistance in Rhopalosiphum padi.
机构信息
State Key Laboratory of Crop Stress Biology for Arid Areas, Key laboratory of Plant Protection Resources and Pest Management of Ministry of Education, Key Laboratory of Integrated Pest Management on the Loess Plateau of Ministry of Agriculture and Rural Affairs, College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi, China.
State Key Laboratory of Crop Stress Biology for Arid Areas, Key laboratory of Plant Protection Resources and Pest Management of Ministry of Education, Key Laboratory of Integrated Pest Management on the Loess Plateau of Ministry of Agriculture and Rural Affairs, College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi, China..
出版信息
Pestic Biochem Physiol. 2023 Aug;194:105528. doi: 10.1016/j.pestbp.2023.105528. Epub 2023 Jul 11.
Uridine diphosphate-glucuronosyltransferases (UGTs) are major multifunctional detoxification phase II enzymes involved in the metabolic detoxification of xenobiotics. However, their roles in insecticides resistance are still unclear. In this study, we identified two UGTs genes (UGT2B13 and UGT2C1) in Rhopalosiphum padi, a serious insect pest of wheat worldwide. Bioassays results showed that the resistance ratio of R. padi resistance strain (LC-R) to lambda-cyhalothrin (LC) was 2963.8 fold. The roles of UGT2B13 and UGT2C1 in lambda-cyhalothrin resistance were evaluated. Results indicated that the UGTs contents were significantly increased in the LC resistant strain of R. padi. UGT2B13 and UGT2C1 were significantly overexpressed in the LC-R strain. Transcription levels of UGT2B13 and UGT2C1 were relatively higher in the gut of LC-R strain. RNA interference (RNAi) of UGT2B13 or UGT2C1 significantly decreased the UGTs contents of the LC-R aphids and increased mortality of R. padi exposure to the LC concentration of LC. This study provides a new view that UGTs are involved in LC resistance of R. padi. The findings will promote further work to detailed the functions of UGTs in the metabolism resistance of insects to insecticides.
尿苷二磷酸葡萄糖醛酸基转移酶（UGTs）是主要的多功能解毒 II 期酶，参与外源性物质的代谢解毒。然而，它们在杀虫剂抗性中的作用尚不清楚。在这项研究中，我们在世界性小麦严重害虫麦长管蚜中鉴定了两个 UGTs 基因（UGT2B13 和 UGT2C1）。生物测定结果表明，麦长管蚜抗性品系（LC-R）对氯氟氰菊酯（LC）的抗性比为 2963.8 倍。评估了 UGT2B13 和 UGT2C1 在氯氟氰菊酯抗性中的作用。结果表明，LC 抗性品系中 UGTs 的含量显著增加。LC-R 品系中 UGT2B13 和 UGT2C1 的表达量显著上调。LC-R 品系肠道中 UGT2B13 和 UGT2C1 的转录水平相对较高。UGT2B13 或 UGT2C1 的 RNA 干扰（RNAi）显著降低了 LC-R 蚜虫中 UGTs 的含量，并增加了 LC 浓度下 R. padi 的死亡率。这项研究提供了一个新的观点，即 UGTs 参与了麦长管蚜对氯氟氰菊酯的抗性。这些发现将促进进一步研究 UGTs 在昆虫对杀虫剂代谢抗性中的功能。
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