Ministry of Agriculture and Rural Affairs Key Laboratory of Sustainable Crop Production in the Middle Reaches of the Yangtze River (Co-construction by Ministry and Province), Hubei Engineering Technology Center for Forewarning and Management of Agricultural and Forestry Pests, College of Agriculture, Yangtze University, Jingzhou, Hubei 434025, China.
Ministry of Agriculture and Rural Affairs Key Laboratory of Sustainable Crop Production in the Middle Reaches of the Yangtze River (Co-construction by Ministry and Province), Hubei Engineering Technology Center for Forewarning and Management of Agricultural and Forestry Pests, College of Agriculture, Yangtze University, Jingzhou, Hubei 434025, China; State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Pestic Biochem Physiol. 2023 Aug;194:105470. doi: 10.1016/j.pestbp.2023.105470. Epub 2023 May 15.
Flavonoids are ubiquitously distributed in plants, showing pleiotropic effects in defense against abiotic and biotic stresses. Although it has been shown that seed priming with flavonoids can enhance plant resistance to abiotic stress, little is known about its potential to enhance plant tolerance to biotic stresses, especially for herbivorous insects. Here, we investigated whether treatment of tomato (Solanum lycopersicum) seeds with rutin improves plant resistance against the whitefly (Bemisia tabaci). Specifically, we measured the effect of rutin seed treatment on tomato seedling vigour, plant growth, feeding behavior and performance of B. tabaci on plants grown from control and rutin-treated seeds, and plant defense responses to B. tabaci attack. We found that seed treatment with different concentrations of rutin (viz 1, 2, 5, 10, and 20 mM) had minimal impact on shoot growth. Furthermore, seed treatment of rutin reduced the developmental rate of nymphs, the fecundity and feeding efficiency of adult females on plants grown from these seeds. The enhanced resistance of tomato against B. tabaci is closely associated with increased flavonoids accumulation, callose deposition and the expression of jasmonic acid (JA)-dependent defense genes. Additionally, callose deposition and expression of JA-dependent genes in tomato plants grown from rutin-treated seeds significantly increased upon B. tabaci infestation. These results suggest that seed treatment with rutin primes tomato resistance against B. tabaci, and are not accompanied by reductions in shoot growth. Defense priming by seed treatments may therefore be suitable for commercial exploitation.
类黄酮广泛分布于植物中，在抵御非生物和生物胁迫方面表现出多种效应。虽然已经表明，用类黄酮对种子进行预处理可以增强植物对非生物胁迫的抗性，但对于生物胁迫，尤其是对于草食性昆虫，其增强植物耐受性的潜力知之甚少。在这里，我们研究了用芦丁处理番茄（Solanum lycopersicum）种子是否可以提高植物对粉虱（Bemisia tabaci）的抗性。具体来说，我们测量了芦丁种子处理对番茄幼苗活力、植物生长、粉虱取食行为和取食表现以及植物对粉虱攻击的防御反应的影响。我们发现，用不同浓度的芦丁（即 1、2、5、10 和 20 mM）处理种子对茎的生长影响最小。此外，芦丁种子处理降低了若虫的发育速度、成虫的产卵量和雌性成虫的取食效率。番茄对粉虱的抗性增强与类黄酮的积累、胼胝质的沉积以及茉莉酸（JA）依赖性防御基因的表达密切相关。此外，在粉虱侵染后，用芦丁处理的种子种植的番茄植物中的胼胝质沉积和 JA 依赖性基因的表达显著增加。这些结果表明，用芦丁处理种子可以使番茄对粉虱产生抗性，而不会降低茎的生长。因此，通过种子处理进行防御诱导可能适合商业开发。
