Department of Plant Physiology and Protein Metabolism Laboratory, University of Osnabruck, Osnabruck, Germany; CellNanOs-Center of Cellular Nanoanalytics, University of Osnabruck, Osnabruck, Germany; Faculty of Biology, University of Osnabruck, Osnabruck, Germany.
Department of Plant Physiology and Protein Metabolism Laboratory, University of Osnabruck, Osnabruck, Germany; CellNanOs-Center of Cellular Nanoanalytics, University of Osnabruck, Osnabruck, Germany; Faculty of Biology, University of Osnabruck, Osnabruck, Germany.
Methods Enzymol. 2023;686:125-141. doi: 10.1016/bs.mie.2023.02.015. Epub 2023 Apr 6.
The tobacco etch virus (TEV) protease is widely used in in vitro and in vivo approaches for the removal of affinity tags from fusion proteins or the generation of proteins with a desired N-terminal amino acid. Processing of fusion proteins by the TEV protease can either be achieved by encoding the TEV protease and its recognition site on one construct (self-cleavage) or on two different constructs (co-expression). Here, we compare the efficiency of the self-splitting approach to the co-expression approach.
烟草蚀纹病毒（TEV）蛋白酶广泛应用于体外和体内方法，用于从融合蛋白中去除亲和标签或生成具有所需 N 末端氨基酸的蛋白质。通过 TEV 蛋白酶处理融合蛋白可以通过在一个构建体（自切割）或两个不同的构建体（共表达）上编码 TEV 蛋白酶及其识别位点来实现。在这里，我们比较了自分裂方法和共表达方法的效率。
