State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China;Department of Microbiology, School of Public Health, Cheeloo College of Medicine, Shandong University, Jinan 250100, Shandong, China.
Biomed Environ Sci. 2023 Jul 20;36(7):604-613. doi: 10.3967/bes2023.088.
NCTC11168 is commonly used as a standard strain for flagellar biosynthesis research. In this report, two distinguished phenotypic isolates (CJ1Z, mutant strain, lawn; CJ2S, complemented strain, normal colony) appeared during laboratory passages for NCTC11168.
Phenotypic assessments, including motility plates, transmission electron microscopy, biofilm formation assay, autoagglutination assay, and genome re-sequencing for these two isolates (CJ1Z, mutant strain; CJ2S, complemented strain) were carried out in this study.
Transmission electron microscopy revealed that the flagellum was lost in CJ1Z. Phenotypic assessments and genome sequencing of the two isolates were performed in this study. The capacity for biofilm formation, colony auto-agglutination, and isolate motility was reduced in the mutant CJ1Z. Comparative genomic analysis indicated a unique native nucleotide insertion in flhA (nt, 2154) that caused the I719Y and I720Y mutations and early truncation in flhA.
FlhA has been found to influence the expression of flagella in . To the best of our knowledge, this is the first study to describe the function of the C-terminal of this protein.
NCTC11168 通常被用作鞭毛生物合成研究的标准菌株。在本报告中，NCTC11168 在实验室传代过程中出现了两个明显的表型分离株（CJ1Z，突变株，菌苔；CJ2S，互补株，正常菌落）。
本研究对这两个分离株（CJ1Z，突变株；CJ2S，互补株）进行了表型评估，包括运动平板、透射电子显微镜、生物膜形成测定、自凝集测定和基因组重测序。
透射电子显微镜显示 CJ1Z 的鞭毛丢失。本研究对这两个分离株进行了表型评估和基因组测序。突变株 CJ1Z 的生物膜形成能力、菌落自凝集能力和分离株运动能力降低。比较基因组分析表明，flhA 中存在一个独特的天然核苷酸插入（nt，2154），导致 I719Y 和 I720Y 突变以及 flhA 的早期截断。
已经发现 FlhA 影响 的 flagella 的表达。据我们所知，这是首次描述该蛋白 C 端的功能。
