Clodfelder-Miller Buffie, DeBerry Jennifer J, Ness Timothy J
Department of Anesthesiology and Perioperative Medicine, University of Alabama at Birmingham, Birmingham, AL, UK.
Heliyon. 2023 Jul 20;9(8):e18495. doi: 10.1016/j.heliyon.2023.e18495. eCollection 2023 Aug.
This neuroanatomical study in four, adult, Sprague-Dawley female rats quantified the number of Urothelial (labeled by intravesical DiI dye administration) and Non-Urothelial (labeled by intraparenchymal injection of Fast blue dye) bladder primary afferent neurons (bPANs) located in the T13, L1, L6 and S1 dorsal root ganglia. Additional immunohistochemical labeling using antibodies to detect either Substance P or CGRP further characterized the bPAN samples as peptidergic or non-peptidergic. Cell counts indicated that Urothelial bPANs were more common at the L6/S1 levels and more likely to be identified as peptidergic when compared with bPANs characterized at T13/L1 levels and with Non-Urothelial bPANs. These studies provide additional evidence that at least two distinct neuronal populations, with differing localization of sensory terminals, differing peptide content, and differing projections to the central nervous system, are responsible for bladder sensation.
这项针对4只成年雌性斯普拉格-道利大鼠的神经解剖学研究，对位于T13、L1、L6和S1背根神经节中的膀胱初级传入神经元（bPANs）数量进行了量化，其中尿路上皮细胞（通过膀胱内注入DiI染料标记）和非尿路上皮细胞（通过实质内注射固蓝染料标记）的bPANs数量都被纳入了统计。使用检测P物质或降钙素基因相关肽（CGRP）的抗体进行的额外免疫组织化学标记，进一步将bPAN样本表征为肽能或非肽能。细胞计数表明，与在T13/L1水平表征的bPANs以及非尿路上皮bPANs相比，尿路上皮bPANs在L6/S1水平更为常见，并且更有可能被鉴定为肽能。这些研究提供了额外的证据，表明至少有两个不同的神经元群体，它们具有不同的感觉末梢定位、不同的肽含量以及向中枢神经系统的不同投射，负责膀胱感觉。
