通过两相分配法对大鼠肝细胞膜区域进行分级分离。
Fractionation of rat liver plasma-membrane regions by two-phase partitioning.
作者信息
Gierow P, Sommarin M, Larsson C, Jergil B
出版信息
Biochem J. 1986 May 1;235(3):685-91. doi: 10.1042/bj2350685.
Abstract
Rat liver plasma membranes, enriched in blood-sinusoidal or bile-canalicular regions by differential and sucrose-gradient centrifugation, were further purified by partitioning in an aqueous polymer two-phase system. This method separates membranes according to differences in surface properties rather than size and density. A several-fold increase in the ratio of leucine aminopeptidase (a bile-canalicular marker) and 5'-nucleotidase to asialo-orosomucoid binding (a blood-sinusoidal marker) was obtained in one fraction, whereas another fraction gave a 2-3-fold increase in ratio of blood-sinusoidal to bile-canalicular markers. Furthermore, the markers for both regions of the plasma membrane, as well as markers for Golgi membranes and lysosomes, showed a heterogeneous behaviour on counter-current distribution.
摘要
通过差速离心和蔗糖梯度离心富集于血窦或胆小管区域的大鼠肝质膜，在水性聚合物双相系统中进行分配进一步纯化。该方法根据表面性质的差异而非大小和密度来分离膜。在一个组分中，亮氨酸氨肽酶（一种胆小管标志物）和5'-核苷酸酶与去唾液酸血清类黏蛋白结合（一种血窦标志物）的比例增加了几倍，而在另一个组分中，血窦标志物与胆小管标志物的比例增加了2至3倍。此外，质膜两个区域的标志物以及高尔基体膜和溶酶体的标志物在逆流分配中表现出异质性。
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