Department of Restorative Dentistry and Periodontology, Faculty of Dentistry, Chiang Mai University, Chiang Mai, Thailand.
National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani, Thailand.
J Contemp Dent Pract. 2023 Jun 1;24(6):351-356. doi: 10.5005/jp-journals-10024-3522.
To evaluate the calcium ions (Ca) diffusion of calcium hydroxide-loaded poly(lactic-co-glycolic acid) biodegradable nanoparticles [Ca(OH)-loaded PLGA NPs] compared with conventional Ca(OH) in a simulated external root resorption model using inductively coupled plasma mass spectrometry (ICP-MS).
Thirty human mandibular premolars were prepared by sectioning the root segments to create roots measuring 10 mm from the anatomical apex. The root canals were instrumented and irrigated. The external root surface cavities were created. The specimens were randomly divided into the following three groups: Poly(lactic-co-glycolic acid) (PLGA; control group, = 10), conventional calcium hydroxide [Ca(OH)] (Metapaste, = 10), and Ca(OH)-loaded PLGA NPs [15% Ca(OH), = 10]. The intracanal materials were placed in the root canals, and the teeth were stored in phosphate-buffered saline at 37°C. The release of Ca was measured at 7, 30, and 60 days using ICP-MS.
Both Ca(OH)-loaded PLGA NPs and Metapaste groups exhibited higher levels of Ca release compared to the PLGA group at all time points. During the initial 7-day period, the Ca(OH)-loaded PLGA NPs exhibited a significantly greater release of Ca compared to Metapaste. From day 7 to day 30, Metapaste displayed a significantly higher release of Ca than the Ca(OH)-loaded PLGA NPs, but it experienced a subsequent decline in Ca release after the 30-day period. After the 30-day mark, the Ca(OH)-loaded PLGA NPs once again exhibited a significantly higher release of Ca compared to Metapaste.
The Ca(OH)-loaded PLGA NPs exhibited sustained release of Ca that exceeded conventional Ca(OH), particularly during the first week, demonstrating a greater amount of Ca release.
The utilization of Ca(OH)-loaded PLGA NPs as an intracanal medication for external inflammatory root resorption provided sustained release and had the potential to enhance the efficacy of inhibiting root resorption more effectively than conventional Ca(OH).
采用电感耦合等离子体质谱法（ICP-MS）评价载氢氧化钙聚乳酸-共-羟基乙酸可生物降解纳米粒子[载氢氧化钙 PLGA 纳米粒子（Ca(OH)-loaded PLGA NPs）]与传统氢氧化钙（Ca(OH)）在模拟外部根吸收模型中的钙离子（Ca）扩散。
将 30 颗人下颌前磨牙沿根尖方向截断 10mm 制备根段，根管预备冲洗后，在牙本质表面制备外部根管腔。随机将样本分为三组：聚乳酸-共-羟基乙酸（PLGA；对照组，n=10）、传统氢氧化钙（Metapaste，n=10）和载氢氧化钙 PLGA 纳米粒子（15% Ca(OH)，n=10）。将根管内材料放入根管内，37℃下在磷酸盐缓冲液中储存。用 ICP-MS 测量第 7、30 和 60 天的 Ca 释放量。
在所有时间点，载氢氧化钙 PLGA NPs 和 Metapaste 组的 Ca 释放量均高于 PLGA 组。在最初的 7 天内，载氢氧化钙 PLGA NPs 的 Ca 释放量显著高于 Metapaste。从第 7 天到第 30 天，Metapaste 的 Ca 释放量显著高于载氢氧化钙 PLGA NPs，但在 30 天后，Ca 释放量下降。30 天后，载氢氧化钙 PLGA NPs 的 Ca 释放量再次显著高于 Metapaste。
载氢氧化钙 PLGA NPs 具有持续释放 Ca 的能力，超过了传统的氢氧化钙，特别是在第 1 周内，释放的 Ca 量更多。
将载氢氧化钙 PLGA NPs 作为外部炎性根吸收的根管内药物，具有持续释放的特点，有可能比传统氢氧化钙更有效地增强抑制根吸收的效果。
