Hasinoff B B, Davey J P
Biochem J. 1986 May 1;235(3):891-3. doi: 10.1042/bj2350891.
The kinetics of the action of fumarase on L-malate and fumarate were investigated at constant ionic strength. This was done to evaluate reports that fumarase follows simple Michaelis-Menten kinetics. However, when pH, buffer concentration and ionic strength are all maintained at constant values, the Lineweaver-Burk plots exhibit pronounced downward curvature, characteristic of negative kinetic co-operativity.
在恒定离子强度下研究了延胡索酸酶作用于L-苹果酸和富马酸的动力学。这样做是为了评估有关延胡索酸酶遵循简单米氏动力学的报道。然而，当pH值、缓冲液浓度和离子强度都保持恒定时，Lineweaver-Burk图呈现出明显的向下弯曲，这是负动力学协同性的特征。
