猪心延胡索酸酶在恒定离子强度下确实表现出负动力学协同性。
Pig heart fumarase really does exhibit negative kinetic co-operativity at a constant ionic strength.
作者信息
Hasinoff B B, Davey J P
出版信息
Biochem J. 1986 May 1;235(3):891-3. doi: 10.1042/bj2350891.
Abstract
The kinetics of the action of fumarase on L-malate and fumarate were investigated at constant ionic strength. This was done to evaluate reports that fumarase follows simple Michaelis-Menten kinetics. However, when pH, buffer concentration and ionic strength are all maintained at constant values, the Lineweaver-Burk plots exhibit pronounced downward curvature, characteristic of negative kinetic co-operativity.
摘要
在恒定离子强度下研究了延胡索酸酶作用于L-苹果酸和富马酸的动力学。这样做是为了评估有关延胡索酸酶遵循简单米氏动力学的报道。然而，当pH值、缓冲液浓度和离子强度都保持恒定时，Lineweaver-Burk图呈现出明显的向下弯曲，这是负动力学协同性的特征。
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Proc Natl Acad Sci U S A. 1953 Sep;39(9):895-900. doi: 10.1073/pnas.39.9.895.
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Cooperativity in enzyme function: equilibrium and kinetic aspects.酶功能中的协同性：平衡和动力学方面
Methods Enzymol. 1980;64:139-92. doi: 10.1016/s0076-6879(80)64009-9.
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Biochem J. 1982 May 1;203(2):351-60. doi: 10.1042/bj2030351.
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