Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan, China.
State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, China.
Animal Model Exp Med. 2023 Aug;6(4):375-380. doi: 10.1002/ame2.12329. Epub 2023 Aug 3.
This study aimed to develop a combined model to quantify the net absorption of volatile fatty acids (VFA) in the large intestine (LI) of pigs.
Fifteen female growing pigs (Duroc × Large White × Landrace) were ranked by body weight (30 ± 2.1 kg) on day 0 and assigned to one of three treatments, namely the basal diet containing different crude fiber (CF) levels (LCF: 3.0% CF, MCF: 4.5% CF, and HCF: 6.0% CF). The pigs were implanted with the terminal ileum fistula and the cannulation of the ileal mesenteric vein (IMV), portal vein (PV), and left femoral artery (LFA) from days 6 to 7. [ C]-Labeled VFA and P-aminohippuric acid were constantly perfused into the terminal ileum fistula and the cannulation of the IMV (day 15), respectively. Blood samples were collected from the PV and the LFA during perfusion (5 h), and LI samples were collected.
The net flux of [ C]-acetic acid in the PV was greater for LCF versus MCF (p = 0.045), but no difference was observed in the net flux of [ C]-propionic acid (p = 0.505) and [ C]-butyric acid (p = 0.35) in the PV among treatments. The deposition of [ C]-acetic acid in the LI was greater for LCF versus MCF (p = 0.014), whereas the deposition of [ C]-propionic acid (p = 0.007) and [ C]-butyric acid (p = 0.037) in the LI was greater for LCF versus HCF.
In conclusion, this pig model was found conducive to study the net absorption of VFAs in the LI, and LCF had more net absorption of VFAs in the LI than MCF and HCF.
本研究旨在开发一种综合模型来量化猪大肠（LI）中挥发性脂肪酸（VFA）的净吸收。
15 头雌性生长猪（杜洛克×大白×长白）按体重（30±2.1kg）排序，分为 3 种处理，即含有不同粗饲料（CF）水平的基础日粮（LCF：3.0%CF、MCF：4.5%CF 和 HCF：6.0%CF）。从第 6 天到第 7 天，猪被植入回肠末端瘘管，并对回肠肠系膜静脉（IMV）、门静脉（PV）和左股动脉（LFA）进行插管。[C]标记的 VFA 和 P-氨基马尿酸分别持续灌注到回肠末端瘘管和 IMV 的插管（第 15 天）。在灌注期间（5h）从 PV 和 LFA 采集血液样本，并采集 LI 样本。
LCF 与 MCF 相比，PV 中[C]乙酸的净流量更大（p=0.045），但 PV 中[C]丙酸（p=0.505）和[C]丁酸（p=0.35）的净流量在处理之间没有差异。LCF 与 MCF 相比，LI 中[C]乙酸的沉积量更大（p=0.014），而 LI 中[C]丙酸（p=0.007）和[C]丁酸（p=0.037）的沉积量在 LCF 与 HCF 之间更大。
总之，本猪模型有利于研究 LI 中 VFA 的净吸收，LCF 比 MCF 和 HCF 具有更多的 LI 中 VFA 的净吸收。
