Nichols G E, Borgman C A, Young W W
Biochem Biophys Res Commun. 1986 Aug 14;138(3):1163-9. doi: 10.1016/s0006-291x(86)80404-1.
One model of tight junction structure suggests that lipids might flow from cell to cell within shared exoplasmic membrane leaflets. We tested this proposal by co-culturing two clones of MDCK epithelial cells, which differed in their content of Forssman glycolipid, and then staining by immunofluorescence with rabbit anti-Forssman Ig. In co-cultures grown on glass cover slips and on nitrocellulose filters, positive Forssman staining was restricted to sharply demarcated clusters of cells formed by the Forssman-positive clone. Integrity of tight junctions between the two clones was indicated on cover slips by the presence of individual domes (hemicysts) composed of both clones and on filters by the generation of transepithelial potential differences. These results suggest that glycolipids in the exoplasmic leaflet of cells in a tight epithelium do not flow to adjacent cells.
一种紧密连接结构模型表明，脂质可能在共享的细胞外质膜小叶内从一个细胞流向另一个细胞。我们通过共培养两个MDCK上皮细胞克隆来测试这一假设，这两个克隆的福斯曼糖脂含量不同，然后用兔抗福斯曼Ig进行免疫荧光染色。在玻璃盖玻片和硝酸纤维素滤膜上生长的共培养物中，福斯曼阳性染色仅限于由福斯曼阳性克隆形成的界限分明的细胞簇。在盖玻片上，由两个克隆组成的单个圆顶（半囊肿）表明两个克隆之间紧密连接的完整性；在滤膜上，跨上皮电位差的产生表明了这一点。这些结果表明，紧密上皮细胞中细胞外小叶中的糖脂不会流向相邻细胞。
