Scherrmann J M, Sandouk P, Guedeney X
Clin Chem. 1986 Jan;32(1 Pt 1):97-100.
Digoxin-like immunoreactive substances (DLIS) have been quantified by two different digoxin radioimmunoassays (RIA) in 47 cord sera. The mean DLIS value (in digoxin equivalents) ranged from 0.960 (SD 0.184) to 0.181 (SD 0.104) nmol/L between the two different kits and different lot numbers of the reagents. One of the RIA methods showed an obvious lot-to-lot effect. The use of a longer incubation interval and a higher incubation temperature markedly decreased cross reactions with DLIS. The effect of modifying the incubation conditions in RIA is similar to that described for assays of steroids because the dissociation rates of the immunocomplex play a critical role. Data suggest a specific interaction between DLIS and digoxin antibodies. Control of the incubation conditions is recommended, to decrease or increase the amount of the DLIS in cord serum specimens.
采用两种不同的地高辛放射免疫分析法（RIA）对47份脐血血清中的地高辛样免疫反应物质（DLIS）进行了定量分析。两种不同试剂盒及不同批次试剂之间，DLIS的平均含量（以地高辛当量计）在0.960（标准差0.184）至0.181（标准差0.104）nmol/L之间。其中一种RIA方法显示出明显的批次间差异。延长孵育时间和提高孵育温度可显著降低与DLIS的交叉反应。在RIA中改变孵育条件的效果与甾体类物质检测中所描述的相似，因为免疫复合物的解离速率起着关键作用。数据表明DLIS与地高辛抗体之间存在特异性相互作用。建议控制孵育条件，以减少或增加脐血血清标本中DLIS的含量。
