School of Public Health, Shenyang Medical College, 146 Huanghe North Avenue, Shenyang, 110034, China.
School of Basic Medicine, Shenyang Medical College, 146 Huanghe North Avenue, Shenyang, 110034, China.
Talanta. 2024 Jan 1;266(Pt 1):124967. doi: 10.1016/j.talanta.2023.124967. Epub 2023 Jul 20.
The ability to detect glutathione (GSH) concentrations in human blood offered a simple and non-invasive method to monitor changes associated with cardiovascular diseases, cancers and diabetes. We showed the potential of employing catalytically active protein-directed nanoflower-like artificial nanozymes (apo-TF-MnO NFs) by bio-mineralization method to produce simple and visible colorimetric sensor for GSH. The experiments proved that apo-TF-MnO NFs exhibited peroxidase, catalase- and superoxide dismutase-like activities, but the most notable feature was the excellent peroxidase-like activity, which could efficiently catalyze the oxidation reaction of 3,3',5,5'- tetramethylbenzidine (TMB) in the existence of hydrogen peroxide (HO) to generate a blue product. Some outcomes also indicated that the apo-TF-MnO NFs had stronger peroxidase-like activity, which was proved by the Michaelis-Menten constant (K) and maximum initial velocity (V). Hence, we used the peroxidase-like activity to develop a GSH colorimetric biosensor. Fortunately, the colorimetric platform exhibited a sensitive response to HO and GSH in the range of 5 μМ to 300 μМ and 0.5 μМ to 35 μМ with a limit of detection of 3.29 μM and 0.15 μM (S/N = 3) under optimal conditions. The feasibility of the simple method was confirmed by qualitative detection of HO and GSH in blood samples from acute coronary syndrome patients. A key outcome of our study was the ability to realized differential identification of GSH for acute coronary syndrome and healthy human without invasive treatment which was an advantage over other methods. This work not only proposed a new type of nanozymes, but also showed the multiple advantages of the apo-TF-MnO NFs for the construction of biosensors. Thus, we believe that apo-TF-MnO NFs with strong peroxidase-like activity can be employed as nanozymes and be widely applied in the fields of medicine and biological sensors.
检测人血液中谷胱甘肽 (GSH) 浓度提供了一种简单且非侵入性的方法，可用于监测与心血管疾病、癌症和糖尿病相关的变化。我们通过生物矿化方法展示了使用具有催化活性的蛋白导向纳米花状人工纳米酶（apo-TF-MnO NFs）的潜力，以产生用于 GSH 的简单可见比色传感器。实验证明，apo-TF-MnO NFs 表现出过氧化物酶、过氧化氢酶和超氧化物歧化酶样活性，但最显著的特征是具有优异的过氧化物酶样活性，它可以在存在过氧化氢 (HO) 的情况下有效地催化 3,3',5,5'-四甲基联苯胺 (TMB) 的氧化反应，生成蓝色产物。一些结果还表明，apo-TF-MnO NFs 具有更强的过氧化物酶样活性，这一点通过米氏常数 (K) 和最大初始速度 (V) 得到了证明。因此，我们利用过氧化物酶样活性开发了一种 GSH 比色生物传感器。幸运的是，比色平台在最佳条件下对 HO 和 GSH 的检测范围分别为 5 μΜ 至 300 μΜ 和 0.5 μΜ 至 35 μΜ，检测限分别为 3.29 μM 和 0.15 μM（S/N = 3），表现出对 HO 和 GSH 的敏感响应。通过对急性冠状动脉综合征患者血液样本中 HO 和 GSH 的定性检测，证实了该简单方法的可行性。我们研究的一个关键结果是，无需侵入性治疗即可实现对急性冠状动脉综合征和健康人 GSH 的差异识别，这是优于其他方法的优势。这项工作不仅提出了一种新型纳米酶，还展示了 apo-TF-MnO NFs 用于构建生物传感器的多种优势。因此，我们相信具有强过氧化物酶样活性的 apo-TF-MnO NFs 可以用作纳米酶，并广泛应用于医学和生物传感器领域。
