Department of Biopharmaceutics and Pharmacodynamics, Medical University of Gdańsk, Al. Gen. Hallera 107, 80-416 Gdańsk, Poland.
Institute of Veterinary Medicine, Nicolaus Copernicus University in Toruń, Gagarina 7, 87-100 Toruń, Poland; Department of Plant Physiology, Genetics, and Biotechnology, University of Warmia and Mazury in Olsztyn, 10-229 Olsztyn, Poland.
J Pharm Biomed Anal. 2023 Oct 25;235:115602. doi: 10.1016/j.jpba.2023.115602. Epub 2023 Jul 25.
Despite a large number of studies, the pathogenesis of polycystic ovary syndrome (PCOS) still remains unexplained. In light of ambiguous observations reported in metabolomics, there is a need to carry out studies focusing on confirming the discriminating power of the proposed metabolomics biomarkers. Our research aimed to perform a validation study of metabolites detected in our previous study from serum samples, on the new set of samples obtained from PCOS women and healthy controls to confirm previously selected compounds. Additionally, the second biological matrix - urine - was used to get a more comprehensive insight into metabolic alterations. We applied two analytical techniques - gas chromatography and liquid chromatography coupled with mass spectrometry to analyze both serum and urine samples obtained from 35 PCOS patients and 35 healthy women. Thank to our approach, we identified and described a comprehensive set of metabolites altered in PCOS patients. Results of our study indicate increased steroid hormone synthesis, alteration in sphingo- and phospholipids metabolism, and disturbed fatty acids metabolism. Moreover, the citric acid cycle, γ-glutamyl cycle, vitamin B metabolism, and a few primary amino acids like tryptophan, phenylalanine, histidine, and alanine are altered.
尽管进行了大量研究，多囊卵巢综合征（PCOS）的发病机制仍未得到解释。鉴于代谢组学中报道的观察结果不明确，有必要开展研究，重点确认所提出的代谢组学生物标志物的区分能力。我们的研究旨在对我们之前研究中从血清样本中检测到的代谢物进行验证研究，使用新的一组来自 PCOS 女性和健康对照者的样本，以确认之前选择的化合物。此外，还使用第二生物基质——尿液，更全面地了解代谢变化。我们应用了两种分析技术——气相色谱和液相色谱与质谱联用，分析了来自 35 名 PCOS 患者和 35 名健康女性的血清和尿液样本。通过我们的方法，我们确定并描述了 PCOS 患者中改变的一整套代谢物。我们的研究结果表明，甾体激素合成增加，鞘脂和磷脂代谢改变，以及脂肪酸代谢紊乱。此外，柠檬酸循环、γ-谷氨酰循环、维生素 B 代谢以及一些必需氨基酸如色氨酸、苯丙氨酸、组氨酸和丙氨酸也发生了改变。
