Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan, 430062, China.
Agricultural College, Guizhou University, Guiyang, 550025, China.
Plant Physiol Biochem. 2023 Sep;202:107915. doi: 10.1016/j.plaphy.2023.107915. Epub 2023 Jul 26.
Kunitz-like protease inhibitors (KTIs) have been identified to play critical roles in insect defense, but evidence for their involvement in drought stress is sparse. The aim of this study was to identify and functionally characterize a Kunitz-like protease inhibitor, GsKTI, from the wild soybean (Glycine soja) variety ED059. Expression patterns suggest that drought stress and insect herbivory may induce GsKTI transcript levels. Transgenic Arabidopsis lines overexpressing GsKTI have been shown to exhibit enhanced drought tolerance by regulating the ABA signaling pathway and increasing xylem cell number. Transgenic Arabidopsis leaves overexpressing GsKTI interfered with insect digestion and thus had a negative effect on the growth of Helicoverpa armigera. It is concluded that GsKTI increases resistance to drought stress and insect attack in transgenic Arabidopsis lines.
Kunitz 蛋白酶抑制剂（KTIs）在昆虫防御中发挥着关键作用，但它们在干旱胁迫中的作用证据很少。本研究旨在从野生大豆（Glycine soja）品种 ED059 中鉴定和功能表征一种 Kunitz 蛋白酶抑制剂 GsKTI。表达模式表明，干旱胁迫和昆虫取食可能诱导 GsKTI 转录水平。过表达 GsKTI 的转基因拟南芥系通过调节 ABA 信号通路和增加木质部细胞数量来表现出增强的耐旱性。过表达 GsKTI 的转基因拟南芥叶片干扰昆虫消化，因此对棉铃虫的生长产生负面影响。结论是，GsKTI 增加了转基因拟南芥系对干旱胁迫和昆虫攻击的抗性。
