疟原虫恶性疟原虫感染与卡波西肉瘤相关疱疹病毒（KSHV）血清阳性率增加及更高的 KSHV 抗体多样性和幅度相关：来自乌干达农村的一项病例对照研究结果。

Plasmodium falciparum Malaria Is Associated With Increased Kaposi Sarcoma-Associated Herpesvirus (KSHV) Seropositivity and Higher KSHV Antibody Breadth and Magnitude: Results of a Case-Control Study From Rural Uganda.

机构信息

Department of Immunology and Microbiology, University of Colorado, Anschutz Medical Campus, Aurora, Colorado.

Cancer Epidemiology Programme, Medical Research Council/Uganda Virus Research Institute and London School of Hygiene and Tropical Medicine Uganda Research Unit, Entebbe, Uganda.

出版信息

J Infect Dis. 2024 Feb 14;229(2):432-442. doi: 10.1093/infdis/jiad308.

DOI:10.1093/infdis/jiad308

PMID:37536670

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10873168/

Abstract

BACKGROUND

Previously, we showed that children with asymptomatic Plasmodium falciparum (Pf) malaria infection had higher Kaposi sarcoma-associated herpesvirus (KSHV) viral load, increased risk of KSHV seropositivity, and higher KSHV antibody levels. We hypothesize that clinical malaria has an even larger association with KSHV seropositivity. In the current study, we investigated the association between clinical malaria and KSHV seropositivity and antibody levels.

METHODS

Between December 2020 and March 2022, sick children (aged 5-10 years) presenting at a clinic in Uganda were enrolled in a case-control study. Pf was detected using malaria rapid diagnostic tests (RDTs) and subsequently with quantitative real-time polymerase chain reaction (qPCR). Children with malaria were categorized into 2 groups: RDT+/PfPCR+ and RDT-/PfPCR+.

RESULTS

The seropositivity of KSHV was 60% (47/78) among Pf-uninfected children, 79% (61/77) among children who were RDT-/PfPCR+ (odds ratio [OR], 2.41 [95% confidence interval {CI}, 1.15-5.02]), and 95% (141/149) in children who were RDT+/PfPCR+ (OR, 10.52 [95% CI, 4.17-26.58]; Ptrend < .001). Furthermore, RDT+/PfPCR+ children followed by RDT-/PfPCR+ children had higher KSHV IgG and IgM antibody levels and reacted to more KSHV antigens compared to uninfected children.

CONCLUSIONS

Clinical malaria is associated with both increased KSHV seropositivity and antibody magnitude, suggesting that Pf is affecting KSHV immunity.

摘要

背景

此前，我们发现无症状恶性疟原虫（Pf）疟疾感染患儿的卡波西肉瘤相关疱疹病毒（KSHV）病毒载量更高，KSHV 血清阳性率更高，KSHV 抗体水平更高。我们假设临床疟疾与 KSHV 血清阳性率之间存在更大的关联。在本研究中，我们研究了临床疟疾与 KSHV 血清阳性率和抗体水平之间的关系。

方法

在 2020 年 12 月至 2022 年 3 月期间，乌干达一家诊所收治的患病儿童（年龄为 5-10 岁）参加了一项病例对照研究。使用疟疾快速诊断检测（RDT）和定量实时聚合酶链反应（qPCR）检测 Pf。将患有疟疾的儿童分为 2 组：RDT+/PfPCR+和 RDT-/PfPCR+。

结果

Pf 未感染儿童的 KSHV 血清阳性率为 60%（47/78），RDT-/PfPCR+儿童为 79%（61/77）（比值比 [OR]，2.41 [95%置信区间 {CI}，1.15-5.02]），RDT+/PfPCR+儿童为 95%（141/149）（OR，10.52 [95% CI，4.17-26.58]；P 趋势 <.001）。此外，与未感染儿童相比，RDT+/PfPCR+儿童随后是 RDT-/PfPCR+儿童的 KSHV IgG 和 IgM 抗体水平更高，对更多的 KSHV 抗原反应。