通过原位杂交检测促肾上腺皮质激素原mRNA的特征。

Characterization of proopiomelanocortin mRNA detected by in situ hybridization.

作者信息

Kelsey J E, Watson S J, Burke S, Akil H, Roberts J L

出版信息

J Neurosci. 1986 Jan;6(1):38-42. doi: 10.1523/JNEUROSCI.06-01-00038.1986.

DOI:10.1523/JNEUROSCI.06-01-00038.1986

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6568624/

Abstract

In situ hybridization is a method for detecting specific nucleotide sequences by using a labeled complementary nucleic acid probe. We have characterized in situ hybridization in rat pituitary using a cDNA probe directed against a portion of proopiomelanocortin (POMC) mRNA to show its specificity in tissue sections. This characterization includes the size of the target mRNA from tissue processed for in situ hybridization, the dissociation pattern of the cDNA:mRNA hybrid, and quantitation of changes in POMC mRNA levels in the rat pituitary intermediate lobe detected both by in situ hybridization and "dot blot" techniques.

摘要

原位杂交是一种通过使用标记的互补核酸探针来检测特定核苷酸序列的方法。我们利用针对阿黑皮素原（POMC）mRNA一部分的cDNA探针，对大鼠垂体中的原位杂交进行了表征，以显示其在组织切片中的特异性。这种表征包括用于原位杂交的组织中靶mRNA的大小、cDNA:mRNA杂交体的解离模式，以及通过原位杂交和“斑点印迹”技术检测到的大鼠垂体中间叶中POMC mRNA水平变化的定量分析。

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