Suppr超能文献

通过原位杂交检测促肾上腺皮质激素原mRNA的特征。

Characterization of proopiomelanocortin mRNA detected by in situ hybridization.

作者信息

Kelsey J E, Watson S J, Burke S, Akil H, Roberts J L

出版信息

J Neurosci. 1986 Jan;6(1):38-42. doi: 10.1523/JNEUROSCI.06-01-00038.1986.

DOI:10.1523/JNEUROSCI.06-01-00038.1986
PMID:3753720
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6568624/
Abstract

In situ hybridization is a method for detecting specific nucleotide sequences by using a labeled complementary nucleic acid probe. We have characterized in situ hybridization in rat pituitary using a cDNA probe directed against a portion of proopiomelanocortin (POMC) mRNA to show its specificity in tissue sections. This characterization includes the size of the target mRNA from tissue processed for in situ hybridization, the dissociation pattern of the cDNA:mRNA hybrid, and quantitation of changes in POMC mRNA levels in the rat pituitary intermediate lobe detected both by in situ hybridization and "dot blot" techniques.

摘要

原位杂交是一种通过使用标记的互补核酸探针来检测特定核苷酸序列的方法。我们利用针对阿黑皮素原（POMC）mRNA一部分的cDNA探针，对大鼠垂体中的原位杂交进行了表征，以显示其在组织切片中的特异性。这种表征包括用于原位杂交的组织中靶mRNA的大小、cDNA:mRNA杂交体的解离模式，以及通过原位杂交和“斑点印迹”技术检测到的大鼠垂体中间叶中POMC mRNA水平变化的定量分析。

相似文献

1
Characterization of proopiomelanocortin mRNA detected by in situ hybridization.通过原位杂交检测促肾上腺皮质激素原mRNA的特征。
J Neurosci. 1986 Jan;6(1):38-42. doi: 10.1523/JNEUROSCI.06-01-00038.1986.
2
[Cloning of DNA complementary to mRNA for proopiomelanocortin from the bovine, rat and human hypophysis. Hormonal regulation of proopiomelanocortin mRNA in the rat hypophysis].
Biokhimiia. 1987 May;52(5):707-14.
3
Detection of proopiomelanocortin mRNA by in situ hybridization with an oligonucleotide probe.用寡核苷酸探针原位杂交法检测阿黑皮素原mRNA。
Proc Natl Acad Sci U S A. 1986 Aug;83(15):5419-23. doi: 10.1073/pnas.83.15.5419.
4
In situ hybridization detection of marked differences in pre-proopiomelanocortin messenger ribonucleic acid content of individual corticotropes and melanotropes.原位杂交检测促肾上腺皮质激素原信使核糖核酸在各个促肾上腺皮质激素细胞和促黑素细胞中含量的显著差异。
Endocrinology. 1989 Mar;124(3):1359-64. doi: 10.1210/endo-124-3-1359.
5
Quantitative in-situ hybridization histochemistry in the rat pituitary gland: effect of bromocriptine on prolactin and pro-opiomelanocortin gene expression.大鼠垂体的定量原位杂交组织化学：溴隐亭对催乳素和阿黑皮素原基因表达的影响。
J Endocrinol. 1988 Aug;118(2):205-10. doi: 10.1677/joe.0.1180205.
6
Histological evaluation of the dopaminergic regulation of proopiomelanocortin gene expression in the intermediate lobe of the rat pituitary, involving in situ hybridization and [3H]thymidine uptake measurement.大鼠垂体中间叶中阿片促黑素皮质素原基因表达的多巴胺能调节的组织学评估，包括原位杂交和[3H]胸苷摄取测量。
Endocrinology. 1987 Mar;120(3):1201-11. doi: 10.1210/endo-120-3-1201.
7
Role of dopamine in the regulation of proopiomelanocortin (POMC) mRNA levels in the arcuate nucleus and pituitary gland of the female rat as studied by in situ hybridization.通过原位杂交研究多巴胺在雌性大鼠弓状核和垂体中对阿黑皮素原（POMC）mRNA水平的调节作用。
Brain Res Mol Brain Res. 1992 Sep;15(1-2):27-32. doi: 10.1016/0169-328x(92)90147-4.
8
Analysis of proopiomelanocortin gene expression during prenatal development of the rat pituitary gland.大鼠垂体产前发育过程中阿片促黑皮质素原基因表达的分析。
Mol Endocrinol. 1989 Aug;3(8):1313-24. doi: 10.1210/mend-3-8-1313.
9
Ontogeny of proopiomelanocortin (POMC) gene expression in the intermediate lobe of the rat pituitary gland.
Neuropeptides. 1990 Aug;16(4):173-9. doi: 10.1016/0143-4179(90)90059-8.
10
In situ transcription: specific synthesis of complementary DNA in fixed tissue sections.原位转录：在固定组织切片中特异性合成互补DNA。
Science. 1988 Jun 17;240(4859):1661-4. doi: 10.1126/science.2454508.

引用本文的文献

1
Fecal dysbiosis in infants with cystic fibrosis is associated with early linear growth failure.囊性纤维化患儿粪便菌群失调与早期线性生长发育不良有关。
Nat Med. 2020 Feb;26(2):215-221. doi: 10.1038/s41591-019-0714-x. Epub 2020 Jan 20.
2
Detection of proopiomelanocortin mRNA by in situ hybridization with an oligonucleotide probe.用寡核苷酸探针原位杂交法检测阿黑皮素原mRNA。
Proc Natl Acad Sci U S A. 1986 Aug;83(15):5419-23. doi: 10.1073/pnas.83.15.5419.
3
Thyroid hormones regulate levels of thyrotropin-releasing-hormone mRNA in the paraventricular nucleus.甲状腺激素调节室旁核中促甲状腺激素释放激素信使核糖核酸的水平。
Proc Natl Acad Sci U S A. 1987 Oct;84(20):7329-33. doi: 10.1073/pnas.84.20.7329.
4
Human growth hormone and prolactin secreting pituitary adenomas analyzed by in situ hybridization.通过原位杂交分析的分泌人生长激素和催乳素的垂体腺瘤
Am J Pathol. 1989 Mar;134(3):605-13.
5
The effects of estrogens on tumor growth and on prolactin and growth hormone mRNA expression in rat pituitary tissues.雌激素对大鼠垂体组织中肿瘤生长以及催乳素和生长激素mRNA表达的影响。
Am J Pathol. 1988 Nov;133(2):397-406.
6
Analysis of endocrine active and clinically silent corticotropic adenomas by in situ hybridization.通过原位杂交分析内分泌活性及临床无症状的促肾上腺皮质激素腺瘤
Am J Pathol. 1990 Aug;137(2):479-88.
7
Neuropeptide gene expression and neural activity: assessing a working hypothesis in nucleus caudalis and dorsal horn neurons expressing preproenkephalin and preprodynorphin.神经肽基因表达与神经活动：评估在表达脑啡肽原和强啡肽原的尾状核和背角神经元中的一个工作假说。
Cell Mol Neurobiol. 1990 Mar;10(1):73-98. doi: 10.1007/BF00733637.
8
In situ hybridization study of pro-opiomelanocortin (POMC) gene expression in human pituitary corticotrophs and their adenomas.人垂体促肾上腺皮质激素细胞及其腺瘤中阿黑皮素原（POMC）基因表达的原位杂交研究
Virchows Arch A Pathol Anat Histopathol. 1991;419(2):107-13. doi: 10.1007/BF01600224.

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验