Aranda F J, Gómez-Fernandez J C
Biochem Int. 1986 Jan;12(1):137-43.
Ubiquinone-10 and ubiquinol-10 were incorporated into dipalmitoylphosphatidylcholine vesicles and their interaction with the phospholipids was monitored by fluorescence measurements of diphenylhexatriene used as a probe. It was found that ubiquinone-10 did not perturb the phospholipid thermotropic pretransition but ubiquinol-10 was able to do so. Although, in ethanolic solution, ubiquinone-10 was a better quencher of diphenylhexatriene than ubiquinol-10, when incorporated into phospholipid multibilayers and at temperatures above Tc, ubiquinone-10 produced a smaller decrease in the intensity of the fluorescence probe than ubiquinol-10. Furthermore, the fluorescence anisotropy of the probe was significantly increased by ubiquinol-10 but not by ubiquinone-10. It was concluded that both forms of coenzyme Q have different localizations in the phospholipid bilayer.
将泛醌-10和泛醇-10掺入二棕榈酰磷脂酰胆碱囊泡中，并通过用作探针的二苯基己三烯的荧光测量来监测它们与磷脂的相互作用。结果发现，泛醌-10不会干扰磷脂的热致预转变，但泛醇-10能够做到这一点。尽管在乙醇溶液中，泛醌-10比泛醇-10是更好的二苯基己三烯猝灭剂，但当掺入磷脂多层膜中且温度高于Tc时，泛醌-10使荧光探针强度的降低比泛醇-10小。此外，探针的荧光各向异性被泛醇-10显著增加，但未被泛醌-10增加。得出的结论是，辅酶Q的两种形式在磷脂双层中具有不同的定位。
