Shapiro H M, Stephens S
Cytometry. 1986 Jan;7(1):107-10. doi: 10.1002/cyto.990070118.
The laser dyes oxazine 750 (OX750), LD700, and rhodamine 800 (R800) can be used in an instrument employing a low-power helium-neon laser source for flow cytometry of DNA content in ethanol-fixed or detergent-permeabilized cells. Cells in near-isotonic medium are stained with 10-30 microM dye, and fluorescence excited at 633 nm is measured at wavelengths above 665 nm. The dyes do not appear to stain RNA, and the intensity of DNA staining is not changed when 2 microM Hoechst 33342 is added to cells simultaneously with a red-excited dye. The effects on fluorescence of addition of DNA to LD700 or R800 in aqueous solution are strongly influenced by the base composition of the DNA; binding mechanisms remain to be determined.
激光染料恶嗪750（OX750）、LD700和罗丹明800（R800）可用于一种采用低功率氦氖激光源的仪器中，用于对乙醇固定或去污剂通透处理的细胞中的DNA含量进行流式细胞术分析。在近等渗培养基中的细胞用10 - 30微摩尔的染料进行染色，并在633纳米激发的荧光在波长665纳米以上进行测量。这些染料似乎不会对RNA进行染色，并且当向细胞中同时加入2微摩尔的Hoechst 33342和一种红色激发染料时，DNA染色强度不会改变。向水溶液中的LD700或R800中添加DNA对荧光的影响受到DNA碱基组成的强烈影响；结合机制仍有待确定。
