Department of Neurosurgery, The First Hospital of Lanzhou University, Lanzhou, Gansu 730000, China.
Department of Oncological Surgery, The First Hospital of Lanzhou University, Lanzhou, Gansu 730000, China.
Aging (Albany NY). 2023 Aug 3;15(15):7440-7450. doi: 10.18632/aging.204907.
Glioma is a general neurological tumor and circular RNAs (circRNAs) have been implicated in glioma development. However, the underlying mechanisms and circRNA biological functions responsible for the regulation of glioma progression remain unknown. In this study, we employ next-generation sequencing (NGS) to investigate altered circRNA expression in glioma tissues. Regulatory mechanisms were studied using luciferase reporter analyses, transwell migration, CCK8, and EdU analysis. Tumorigenesis and metastasis assays were utilized to determine the function of hsa_circ_0010889 in glioma. Our results showed that hsa_circ_0010889 expression increased in glioma cell lines and tissues, indicating that hsa_circ_0010889 may be involved in glioma progression. Downregulation of hsa_circ_0010889 inhibited glioma invasion and proliferation in both and experiments and luciferase report assays found that miR-590-5p and SATB1 were downstream targets for hsa_circ_0010889. SATB1 overexpression or miR-590-5p inhibition reversed glioma cells proliferation and migration post-silencing of hsa_circ_0010889. Taken together, our study demonstrates that hsa_circ_0010889 downregulation inhibits glioma progression through the miR-590-5p/SATB1 axis.
神经胶质瘤是一种常见的神经肿瘤，环状 RNA（circRNAs）被认为与神经胶质瘤的发生有关。然而，负责调节神经胶质瘤进展的潜在机制和 circRNA 生物学功能仍不清楚。在这项研究中，我们采用下一代测序（NGS）来研究神经胶质瘤组织中circRNA 表达的变化。通过荧光素酶报告分析、transwell 迁移、CCK8 和 EdU 分析研究了调节机制。利用肿瘤发生和转移测定来确定 hsa_circ_0010889 在神经胶质瘤中的功能。我们的研究结果表明，hsa_circ_0010889 在神经胶质瘤细胞系和组织中表达增加，表明 hsa_circ_0010889 可能参与神经胶质瘤的进展。下调 hsa_circ_0010889 抑制了 和 实验中的神经胶质瘤侵袭和增殖，荧光素酶报告实验发现 miR-590-5p 和 SATB1 是 hsa_circ_0010889 的下游靶点。SATB1 过表达或 miR-590-5p 抑制逆转了 hsa_circ_0010889 沉默后神经胶质瘤细胞的增殖和迁移。总之，我们的研究表明 hsa_circ_0010889 的下调通过 miR-590-5p/SATB1 轴抑制神经胶质瘤的进展。
