拟南芥和水稻花药形态建成和花粉活力的细胞生物学分析。
Cell Biological Analyses of Anther Morphogenesis and Pollen Viability in Arabidopsis and Rice.
机构信息
State Key Laboratory of Genetic Engineering, Ministry of Education Key Laboratory of Biodiversity Sciences and Ecological Engineering and Institute of Biodiversity Sciences, Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai, China.
School of Life Sciences, East China Normal University, Shanghai, China.
出版信息
Methods Mol Biol. 2023;2686:199-218. doi: 10.1007/978-1-0716-3299-4_8.
Major advances have been made in our understanding of anther developmental processes in flowering plants through a combination of genetic studies, cell biological technologies, biochemical analyses, microarray and high-throughput sequencing-based approaches. In this chapter, we summarize widely used protocols for pollen viability staining, investigation of anther morphogenesis by scanning electron microscopy (SEM), light microscopy of semi-thin sections, ultrathin section-based transmission electron microscopy (TEM), TUNEL (terminal deoxynucleotidyl transferase-mediated 2'-deoxyuridine 5'-triphosphate (dUTP) nick end labeling) assay for tapetum programmed cell death, and laser microdissection procedures to obtain specific cells or cell layers for transcriptome analysis.
通过遗传研究、细胞生物学技术、生物化学分析、基于微阵列和高通量测序的方法相结合，我们对开花植物花粉囊发育过程有了更深入的理解。在本章中，我们总结了广泛使用的花粉活力染色、扫描电子显微镜（SEM）研究花粉囊形态发生、半薄切片的光学显微镜、基于超薄切片的透射电子显微镜（TEM）、TUNEL（末端脱氧核苷酸转移酶介导的 2'-脱氧尿苷 5'-三磷酸（dUTP）缺口末端标记）法用于绒毡层程序性细胞死亡的检测，以及激光显微切割程序，以获得特定的细胞或细胞层进行转录组分析。
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