College of Agriculture, Nanjing Agricultural University, No. 1 Weigang, Nanjing 210095, People's Republic of China.
Rice Research Institute, Jiangxi Academy of Agricultural Sciences, No. 602 Nanlian Road, Nanchang 330200, People's Republic of China.
Plant Cell Physiol. 2023 Oct 16;64(10):1146-1158. doi: 10.1093/pcp/pcad078.
Auxin plays an essential role in modulating leaf development. However, its role in leaf development in rice (Oryza sativa L.) remains largely unknown. In this study, we found that PINOID (OsPID) and two Sister-of-PIN1s, termed PIN-FORMED1c (OsPIN1c) and OsPIN1d, are necessary for rice leaf development. The ospin1c ospin1d null mutant lines presented severe defects in leaf morphogenesis, including drooping and semi-drooping blades, an abnormally thickened sheath and lamina joint, and fused leaves with absent ligules and auricles. Loss-of-function ospid mutants displayed generally similar leaf morphology but lacked leaf fusion. Interestingly, misshaped leaf genesis displayed a preference for being ipsilateral. In addition, OsPIN1c and OsPID were commonly localized in the initiating leaf primordia. Furthermore, accompanied by the more severe organ morphogenesis in the ospin1c ospin1d ospid triple mutant, RNA sequencing analysis revealed that many genes essential for leaf development have an altered expression level. Together, this study furthers our understanding of the role auxin transport plays during leaf development in monocot rice.
生长素在调节叶片发育中起着至关重要的作用。然而，生长素在水稻（Oryza sativa L.）叶片发育中的作用在很大程度上仍然未知。在这项研究中，我们发现 PINOID（OsPID）和两个 Sister-of-PIN1，称为 PIN-FORMED1c（OsPIN1c）和 OsPIN1d，是水稻叶片发育所必需的。ospin1c ospin1d 缺失突变体系表现出叶片形态发生的严重缺陷，包括叶片下垂和半下垂、鞘和叶片联合的异常增厚以及无叶舌和耳的叶片融合。ospid 功能丧失突变体表现出一般相似的叶片形态，但缺乏叶片融合。有趣的是，畸形叶片的发生偏向于同侧。此外，OsPIN1c 和 OsPID 通常定位于起始叶片原基中。此外，在 ospin1c ospin1d ospid 三重突变体中，器官形态发生更为严重，RNA 测序分析表明，许多对叶片发育至关重要的基因的表达水平发生了改变。总之，这项研究进一步加深了我们对生长素在单子叶水稻叶片发育中的作用的理解。
