Department of Prosthodontics, Affiliated Stomatology Hospital of Nanjing Medical University; Jiangsu Province Key Laboratory of Oral Diseases, Jiangsu Province Engineering Research Center of Stomatological Translational Medicine, Nanjing 210029, China.
Department of Prosthodontics, Affiliated Stomatology Hospital of Nanjing Medical University; Jiangsu Province Key Laboratory of Oral Diseases, Jiangsu Province Engineering Research Center of Stomatological Translational Medicine, Nanjing 210029, China.
Colloids Surf B Biointerfaces. 2023 Oct;230:113484. doi: 10.1016/j.colsurfb.2023.113484. Epub 2023 Jul 29.
Poor osteogenesis caused by limited bioactivity and peri-implantitis caused by bacterial colonization are the main challenges affecting the use of zirconia-based materials in dental implants. Accordingly, the development of a surface treatment method with an antibacterial effect and that promotes osteogenesis without damage to cells is crucial for yttrium-stabilized tetragonal zirconia (Y-TZP) implants. Herein, we have developed a functional surface modification strategy whereby a poly (ethylene imine)/hyaluronic acid /chitosan-chlorogenic acid (PEI/HA/CGA-CS) conjugate is deposited on a zirconia surface by the layer-by-layer (LBL) technique, enhancing its osteogenic differentiation and antibacterial activities. The results showed that the PEI/HA/CGA-CS coating improved the wettability of the zirconia surface and maintained stable release of CGA. The PEI/HA/CGA-CS functional coating was found to promote early cell adhesion, proliferation, differentiation, and calcification. The results of bacterial adhesion and activity tests showed that the coating effectively inhibits the proliferation and activity of Porphyromonas gingivalis (P. gingivalis) and Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) without impairing the biological activity of osteoblasts. In addition, we found that the PEI/HA/CGA-CS coating enhances the osteogenesis of MC3T3-E1 cells by promoting the protein expression of Nephronectin (NPNT) and activating the Wnt/β-catenin signaling pathway. The above results are of profound significance for the practical application of zirconia-based implants. DATA AVAILABILITY: Data will be made available on request.
较差的成骨能力和由细菌定植引起的种植体周围炎是影响氧化锆基材料在牙科种植体中应用的主要挑战。因此，开发一种具有抗菌作用且不会损害细胞的促进成骨作用的表面处理方法对于钇稳定四方氧化锆（Y-TZP）植入物至关重要。在此，我们开发了一种功能表面改性策略，通过层层（LBL）技术将聚（乙二胺）/透明质酸/壳聚糖-绿原酸（PEI/HA/CGA-CS）缀合物沉积在氧化锆表面上，从而增强其成骨分化和抗菌活性。结果表明，PEI/HA/CGA-CS 涂层改善了氧化锆表面的润湿性并保持 CGA 的稳定释放。PEI/HA/CGA-CS 功能涂层被发现可促进早期细胞黏附、增殖、分化和钙化。细菌黏附和活性测试的结果表明，该涂层可有效抑制牙龈卟啉单胞菌（P. gingivalis）和伴放线放线杆菌（A. actinomycetemcomitans）的增殖和活性，而不会损害成骨细胞的生物活性。此外，我们发现 PEI/HA/CGA-CS 涂层通过促进 Nephrontin（NPNT）的蛋白表达和激活 Wnt/β-catenin 信号通路来增强 MC3T3-E1 细胞的成骨作用。上述结果对氧化锆基植入物的实际应用具有重要意义。数据可用性：将根据要求提供数据。
