一种用于 qPCR 土壤和沉积物样品中抑制测试和控制的简单方法。
A simple method for testing and controlling inhibition in soil and sediment samples for qPCR.
机构信息
British Geological Survey, Keyworth, Nottingham NG12 5GG, UK; School of Biosciences, Sutton Bonington, University of Nottingham, Loughborough LE12 5RD, UK.
School of Biosciences, Sutton Bonington, University of Nottingham, Loughborough LE12 5RD, UK.
出版信息
J Microbiol Methods. 2023 Sep;212:106795. doi: 10.1016/j.mimet.2023.106795. Epub 2023 Aug 3.
The presence of polymerase-chain-reaction (PCR) inhibitors in many environmental samples can make reliable and repeatable quantitative-polymerase-chain-reaction (qPCR) analysis difficult without sample dilution. To estimate an optimal sample dilution for qPCR and reduce effects of inhibition, a simple test based on multiple dilution series of samples is presented that avoids the use of internal controls and standards reducing complexity and cost.
许多环境样本中存在聚合酶链反应（PCR）抑制剂，如果不进行样品稀释，就很难进行可靠和可重复的定量聚合酶链反应（qPCR）分析。为了估计 qPCR 的最佳样品稀释度并减少抑制作用的影响，提出了一种基于多个样品稀释系列的简单测试方法，该方法避免了使用内部对照和标准品，从而降低了复杂性和成本。
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