Department of Sports Medicine, The First Affiliated Hospital, Jinan University, Guangzhou 510632, China; Key Laboratory of Eutrophication and Red Tide Prevention of Guangdong Higher Education Institute, College of Life Science and Technology, Jinan University, Guangzhou 510632, China.
School of Laboratory Medicine, Youjiang Medical University for Nationalities, Baise, Guangxi 533000, China.
Bioresour Technol. 2023 Nov;387:129611. doi: 10.1016/j.biortech.2023.129611. Epub 2023 Aug 2.
The scarcity of natural fossil fuels presents a promising opportunity for the development of renewable microalgae-based biofuels. However, the current microalgae cultivation is unable to effectively address the high costs of the production of biofuels. To tackle this challenge, this study focused on recruiting engineered Phaeodactylum tricornutum (FabG-OE) to enhance biomass accumulation and lipid production by employing food waste hydrolysate under temperature variations. The biomass and lipid accumulations of FabG-OE were improved effectively in mixed culture medium and food waste hydrolysate at a volume ratio (v/v) of 80:20 at 30 °C. It was found that oxidative stress might contribute to the overexpression of lipogenic genes, thereby leading to lipogenesis at 30 °C. Upscaling cultivation of FabG-OE at 30 °C using a semi-continuous strategy and batch strategy was conducted to achieve 0.73 and 0.77 g/L/d of biomass containing 0.35 and 0.38 g/L/d of lipid, respectively. In summary, these findings provide valuable insights for advancing microalgae-based biofuel production.
自然资源的匮乏为可再生微藻生物燃料的发展提供了一个有前景的机会。然而，目前的微藻培养技术无法有效地降低生物燃料的生产成本。为了应对这一挑战，本研究聚焦于招募工程化的三角褐指藻（FabG-OE），以利用温度变化下的食物垃圾水解物来提高生物质积累和脂质生产。在混合培养基和食物垃圾水解物（体积比为 80:20）中，FabG-OE 的生物量和脂质积累在 30°C 下得到了有效提高。研究发现，氧化应激可能有助于脂基因的过表达，从而导致 30°C 下的脂生成。采用半连续和分批策略在 30°C 下扩大 FabG-OE 的培养，生物量的产率分别达到 0.73 和 0.77 g/L/d，脂质的产率分别达到 0.35 和 0.38 g/L/d。总之，这些发现为推进微藻生物燃料生产提供了有价值的见解。
