Pamukkale University, Faculty of Medicine, Department of Physiology, Denizli, Turkey.
Pamukkale University, Faculty of Medicine, Department of General Surgery, Denizli, Turkey.
Life Sci. 2023 Oct 1;330:121987. doi: 10.1016/j.lfs.2023.121987. Epub 2023 Aug 2.
We aimed to investigate the time-dependent alterations of serum, adipose tissue WISP1, Nrg4, asprosin, SPX adipokines and serum ER stress markers GRP78, XBP1, ATF6, CHOP in obese patients who underwent laparoscopic sleeve gastrectomy (LSG).
Morbidly obese patients (n = 19) and normal-weight individuals (n = 19) were compared. Preoperative (control, obese) and postoperative 1st, 3rd and 6th month (obese) follow-up measurements were obtained. Levels of adipokines, ER stress markers were measured with commercial kits.
Body mass index (BMI), total fat, trunk fat mass, fat percentage of obese patients decreased after LSG. Postoperative serum glucose, insulin, HOMA-IR, triglyceride levels of obese patients decreased, HDL increased. In obese patients, preoperative LDL and total cholesterol, which were not different from control, were higher in the postoperative 6th month measurements. Omentum WISP1, subcutaneous adipose tissue WISP1 and SPX, and serum WISP1, asprosin, CHOP levels were higher, Nrg4 lower in obese patients. Serum Nrg4 was still lower in the postoperative 1st month measurements, while WISP1 was higher in the 3rd and asprosin in the 3rd, 6th months compared to control. 1st and 3rd month ATF6 and 3rd month CHOP concentrations were lower than preoperative values. Serum CHOP measured at the 6th month was significantly higher than control. Negative correlations were observed between serum Nrg4 and fat percentage, TG concentration. CHOP was negatively correlated with fat percentage.
The correlations between changes in serum Nrg4, CHOP and fat percentage highlight the roles of Nrg4 and CHOP in the fat loss following LSG.
本研究旨在探讨腹腔镜袖状胃切除术（LSG）后肥胖患者血清、脂肪组织 WISP1、Nrg4、Asprosin、SPX 脂肪因子和血清内质网应激标志物 GRP78、XBP1、ATF6、CHOP 的时间依赖性变化。
比较了病态肥胖患者（n=19）和正常体重个体（n=19）。进行了术前（对照组、肥胖组）和术后 1、3、6 个月（肥胖组）的随访测量。采用商业试剂盒测量脂肪因子和内质网应激标志物的水平。
LSG 后肥胖患者的体重指数（BMI）、总脂肪、躯干脂肪质量、脂肪百分比降低。肥胖患者术后血清葡萄糖、胰岛素、HOMA-IR、甘油三酯水平降低，HDL 升高。肥胖患者术前 LDL 和总胆固醇与对照组无差异，但在术后 6 个月测量时较高。肥胖患者的网膜 WISP1、皮下脂肪组织 WISP1 和 SPX 以及血清 WISP1、Asprosin、CHOP 水平较高，Nrg4 水平较低。与对照组相比，肥胖患者术后第 1 个月血清 Nrg4 水平仍较低，第 3 个月 WISP1 水平较高，第 3、6 个月 Asprosin 水平较高。第 1 和第 3 个月 ATF6 和第 3 个月 CHOP 浓度低于术前值。第 6 个月血清 CHOP 测量值明显高于对照组。血清 Nrg4 与脂肪百分比、TG 浓度呈负相关。CHOP 与脂肪百分比呈负相关。
血清 Nrg4 和 CHOP 与脂肪百分比的变化之间的相关性突出了 Nrg4 和 CHOP 在 LSG 后脂肪减少中的作用。
