微小 RNA 表达作为早期子宫内膜癌的诊断参数。

MicroRNA expression as a diagnostic parameter in early endometrial cancer.

机构信息

University of Kragujevac, Faculty of Science, Kragujevac, Serbia

Health Center Raška, Raška, Serbia.

出版信息

Int J Gynecol Cancer. 2023 Sep 4;33(9):1394-1401. doi: 10.1136/ijgc-2023-004579.

DOI:10.1136/ijgc-2023-004579

PMID:37541686

Abstract

OBJECTIVES

MicroRNAs (miRNAs) have emerged as biomarkers that showed strong diagnostic potential in various diseases, including cancer. This study aimed to estimate the expression and diagnostic potential of miRNAs (miR-200a, miR-21, miR-210, miR-126, and miR-130a) in endometrial cancer samples. The DICER1 and AGO2 genes were also analysed.

METHODS

The expression of miRNAs, DICER1, and AGO2 was quantified using the quantitative real-time PCR method in 40 tissue samples with early-stage endometrial cancer and 16 normal controls.

RESULTS

All tested miRNAs showed significantly higher expression in endometrial cancer compared with the control group, while DICER1 was significantly downregulated. The expression levels of miR-200a, miR-21, and miR-210 were negatively correlated with DICER1 expression. Individually, miR-200a, miR-21, miR-210, and DICER1 showed the best diagnostic performance in distinguishing patients with endometrial cancer from normal controls, whereas a combination of all biomarkers resulted in an even higher area under the curve.

CONCLUSIONS

Our study showed that a panel of selected biomarkers (miR-200a, miR-21, miR-210, miR-126, miR-130a, DICER1, and AGO2) may be candidates for the detection of early-stage endometrial cancer.

摘要

目的

微小 RNA（miRNAs）已成为各种疾病（包括癌症）强有力的诊断标志物。本研究旨在评估 miRNA（miR-200a、miR-21、miR-210、miR-126 和 miR-130a）在子宫内膜癌样本中的表达和诊断潜力。还分析了 DICER1 和 AGO2 基因。

方法

采用实时定量 PCR 法检测 40 例早期子宫内膜癌组织样本和 16 例正常对照中 miRNA、DICER1 和 AGO2 的表达情况。

结果

与对照组相比，所有检测到的 miRNA 在子宫内膜癌中表达均显著升高，而 DICER1 表达显著下调。miR-200a、miR-21 和 miR-210 的表达水平与 DICER1 表达呈负相关。单独使用 miR-200a、miR-21、miR-210 和 DICER1 区分子宫内膜癌患者和正常对照的诊断性能最佳，而所有生物标志物的组合则导致曲线下面积更高。